Anti-UQCRC1 Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P31930

Core Product Specifications and Parameters

Biological Background: UQCRC1 Function and Localization

• UQCRC1, also known as Cytochrome b-c1 complex subunit 1 or Core protein I, is a key component of the mitochondrial ubiquinol-cytochrome c oxidoreductase (Complex III), part of the electron transport chain that drives oxidative phosphorylation.
• Complex III catalyzes electron transfer from ubiquinol to cytochrome c and contributes to the proton gradient across the inner mitochondrial membrane through the Q cycle, coupling redox reactions to proton translocation.
• The core subunits UQCRC1 and UQCRC2 share homology with mitochondrial-processing peptidase (MPP) subunits, suggesting an additional role in the proteolytic processing of the Rieske protein (UQCRFS1) for complex III maturation.
• UQCRC1 localizes specifically to the mitochondrial inner membrane, as a fundamental membrane-embedded subunit of the respiratory chain complex.
• The protein is broadly expressed in metabolically active tissues, including brain (substantia nigra, striatum, cortex, cerebellum), spinal cord, heart, kidney, liver, and muscle.
• Post-translational modifications include acetylation and phosphorylation, identified through proteomics studies, which may regulate its function or complex assembly.
• Genetic variations in UQCRC1 have been linked to neurodegenerative diseases; the protein is associated with keywords such as Disease variant, Parkinsonism, and Neuropathy.
• UQCRC1 appears to play a critical role in maintaining proper mitochondrial function in nigral dopaminergic neurons, highlighting its potential relevance in Parkinson’s disease. Related references: PMID:33141179

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment covering amino acids 156–480 of human UQCRC1, expressed as a fusion protein. This region is well-conserved, so the antibody may recognize the target in its native conformation.
• For Western blotting, the predicted molecular weight of human UQCRC1 is approximately 53 kDa (UniProt annotation: 52.6 kDa). Band size may vary slightly across species or due to post-translational modifications.
• When performing IHC-P, standard antigen retrieval methods (e.g., heat-induced epitope retrieval) are recommended, as the epitope may be masked in formalin-fixed paraffin-embedded tissues.
• The antibody is validated for human, mouse, and rat samples. For other species, cross-reactivity should be experimentally confirmed.
• Suitable for multiple immunodetection techniques (WB, IHC-P, IF/ICC, IP, ELISA), but optimal working dilutions should be determined empirically for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and institutes with integrated access to IVD raw materials, technical services, and consulting. This UQCRC1 antibody is part of our comprehensive offering of high-quality antibodies for mitochondrial research and neurodegenerative disease studies. From validated antibody pairs to bulk ancillary reagents and custom rare-target sourcing, we provide scalable solutions to accelerate your assay development and clinical translation.