Anti-UQCR10 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UDW1

Core Product Specifications and Parameters

Biological Background: UQCR10 Function and Localization

• UQCR10 encodes cytochrome b-c1 complex subunit 9, also known as Complex III subunit 9, Complex III subunit X, cytochrome c1 non-heme 7 kDa protein, and ubiquinol-cytochrome c reductase complex 7.2 kDa protein.
• It is a core component of mitochondrial respiratory chain complex III (cytochrome b-c1 complex), which catalyzes electron transfer from ubiquinol to cytochrome c, coupled with proton translocation across the inner mitochondrial membrane via the Q cycle, essential for oxidative phosphorylation.
• Complex III works together with succinate dehydrogenase (complex II) and cytochrome c oxidase (complex IV) to transfer electrons from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives ATP synthesis.
• UQCR10 localizes to the mitochondrion inner membrane and is predicted to contain a transmembrane helix, consistent with its role as a membrane-embedded subunit.
• The protein undergoes alternative splicing and has been directly sequenced at the protein level; its calculated molecular weight is approximately 7.3 kDa.
• Keywords associated with UQCR10 include alternative splicing, electron transport, transmembrane, mitochondrial inner membrane, respiratory chain, and transport.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-63 of human UQCR10, likely recognizing the full-length protein. Consider this when designing downstream assays.
• This antibody is applicable for western blot (detecting a band near 7 kDa), IHC-P, IF/ICC, and ELISA. Optimize dilutions empirically for each assay type.
• For enhanced detection in western blot, mitochondrial fraction enrichment or appropriate sample preparation may improve sensitivity.
• Validate antibody performance in relevant sample systems (human, mouse, rat) based on the documented cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This UQCR10 rabbit polyclonal antibody is part of our comprehensive portfolio of mitochondrial and metabolic research reagents, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare target raw-material sourcing or customized solutions, CamelBio provides tailored procurement services to accelerate your assay development.