Anti-UQCR10 Polyclonal Antibody for WB and ELISA - Q9UDW1

Core Product Specifications and Parameters

Biological Background: UQCR10 Function and Localization

• UQCR10, also known as Cytochrome b-c1 complex subunit 9 (Complex III subunit 9/X, Ubiquinol-cytochrome c reductase 7.2 kDa protein), is encoded by the UQCR10 (UCRC) gene.
• As a core subunit of mitochondrial respiratory chain Complex III (cytochrome b-c1 complex), it participates in electron transfer from ubiquinol to cytochrome c, coupled with proton translocation across the inner mitochondrial membrane.
• The complex operates via the Q cycle mechanism, where UQCR10 contributes to the structural integrity and functional assembly of the 11-subunit complex.
• It localizes exclusively to the mitochondrial inner membrane, as supported by direct protein sequencing and proteomics identification.
• Alternative splicing has been reported for this gene, though the functional significance of isoforms remains to be fully characterized.
• The protein is small, with a molecular weight of approximately 7.3 kDa, and contains transmembrane helices that anchor it in the membrane.
• 3D-structural studies have provided insights into the arrangement of Complex III subunits, including UQCR10.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant protein covering the full-length (1-63) of human UQCR10, which may contribute to recognition of native protein.
• For Western blot, consider using mitochondrial-enriched fractions from human, mouse, or rat tissue/cell lysates, as the target is a low-abundance mitochondrial membrane protein.
• The predicted molecular weight is ~7 kDa; ensure appropriate gel percentage and transfer conditions for small proteins.
• ELISA may be used for titer determination or detection in purified mitochondrial complex samples; due to low molecular weight, sandwich ELISA may require careful antibody pair selection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies for mitochondrial research targets like UQCR10. Whether you require bulk quantities of this polyclonal antibody, optimized antibody pairs for immunoassays, or custom sourcing of ancillary reagents, our supply chain can bridge your development needs from concept to clinic.