Anti-UQCC2 Polyclonal Antibody for WB, IHC-P, ELISA - Q9BRT2

Core Product Specifications and Parameters

Biological Background: UQCC2 Function and Localization

• UQCC2 (Ubiquinol-cytochrome c reductase complex assembly factor 2), also known as M19, Cbp6, MNF1, is a mitochondrial protein encoded by the UQCC2 gene (Gene ID: 84300) on chromosome 6.
• It is essential for the assembly of mitochondrial respiratory chain complex III (cytochrome b-c1 complex), facilitating cytochrome b translation and/or stability.
• UQCC2 modulates respiratory chain activities, affecting oxygen consumption and ATP production, and thereby regulates skeletal muscle differentiation and insulin secretion by pancreatic beta-cells.
• Subcellularly, UQCC2 localizes to the mitochondrial matrix, nucleoid, intermembrane space, and inner membrane, indicating dynamic roles within mitochondria.
• Tissue expression is prominent in pancreas, skeletal muscle, kidney, liver, and heart, consistent with its role in energy metabolism.
• Keywords associated with UQCC2 include membrane, mitochondrion, mitochondrial inner membrane, mitochondrial nucleoid, and primary mitochondrial disease, highlighting its clinical relevance in mitochondrial disorders.
• No known alternative splicing isoforms are documented for UQCC2 according to UniProt.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 14-126, near the N-terminus; consider epitope accessibility in native conformations when designing immunoprecipitation or IF experiments.
• For Western blotting, a predicted band at ~15 kDa is expected; however, additional bands may occur due to post-translational modifications or protein turnover. Validate in mitochondria-enriched fractions.
• Cross-reactivity with mouse and rat UQCC2 is reported; optimize antibody dilution for each species and sample type.
• In IHC-P, citrate buffer antigen retrieval (pH 6.0) is often suitable for mitochondrial targets; test with appropriate controls.
• For ELISA applications, polyclonal antibodies can serve as both capture and detection antibodies; consider pairing with a monoclonal for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for diagnostic manufacturers and research laboratories, offering a comprehensive portfolio of validated IVD raw materials. For mitochondrial research and respiratory chain disorder applications, we supply high-quality antibodies such as this anti-UQCC2 polyclonal antibody, along with optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing solutions for rare and emerging targets. Let CamelBio streamline your assay development from concept to clinic.