Anti-UPP1 Rabbit Polyclonal Antibody for WB and ELISA - Q16831

Core Product Specifications and Parameters

Biological Background: UPP1 Function and Localization

• Protein identity: Uridine phosphorylase 1 (UPP1), also referred to as UP, is encoded by the UPP1 gene (GeneID: 7378) and belongs to the uridine phosphorylase family.
• Enzymatic activity: UPP1 catalyzes the reversible phosphorolysis of uridine to uracil and ribose-1-phosphate, playing a crucial role in pyrimidine salvage and homeostasis. Related references: PMID:7488099
• Substrate specificity: Exhibits broad substrate recognition, also accepting deoxyuridine and structurally related analogs, which may have implications for nucleoside analog prodrug activation.
• Metabolic role: The released ribose-1-phosphate can be utilized as a carbon and energy source or for nucleotide synthesis, linking UPP1 to cellular energy metabolism and proliferation.
• Alternative splicing: Multiple transcript variants are predicted, potentially yielding protein isoforms with distinct regulatory or catalytic properties.
• Structural insights: Experimentally determined 3D structures are available, providing atomic-level details of the active site and substrate binding, which can guide inhibitor design.
• Proteomics evidence: UPP1 has been identified at the protein level in multiple tissues, confirming its expression in human cells.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 89–173, an interior region of UPP1; the antibody is expected to recognize total UPP1 but may not distinguish all isoforms.
• For Western blot, the predicted molecular weight is approximately 34 kDa; a band near this size in human cell or tissue lysates should be monitored.
• In ELISA, consider pairing this polyclonal with a UPP1-specific monoclonal for a sandwich format, or use it as a capture/detection reagent after in-house validation.
• Since polyclonals can exhibit lot-to-lot variability, titrate each new lot in your specific assay system and include proper positive/negative controls.
• Cross-reactivity is confirmed for human; for other species, sequence alignment of the immunogen region is advised before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams with streamlined access to validated IVD raw materials. This anti-UPP1 polyclonal antibody supports investigations into pyrimidine metabolism and cancer biology, and we can further assist with matched antibody pairs, bulk ancillary reagents, and sourcing of rare target materials to accelerate your assay development from concept to clinic.