Anti-UPP1 Rabbit Monoclonal Antibody for WB, ELISA - Q16831

Core Product Specifications and Parameters

Biological Background: Uridine Phosphorylase 1 Function and Localization

• Uridine phosphorylase 1 (UPP1, also known as UPase or UP) is a key enzyme in pyrimidine nucleoside metabolism, catalyzing the reversible phosphorolysis of uridine to uracil and ribose-1-phosphate (PubMed:7488099). Related references: PMID:7488099
• This enzyme can also accept deoxyuridine and other analogous compounds, indicating broad substrate specificity that supports both pyrimidine salvage and energy utilization pathways.
• UPP1 belongs to the glycosyltransferase family, as classified by its transferase activity (UniProt keyword: Glycosyltransferase).
• The gene undergoes alternative splicing, producing multiple transcript variants that may contribute to tissue-specific regulation.
• Structural studies (3D-structure available) have elucidated the catalytic mechanism, providing insights for inhibitor design.
• The molecular weight of the canonical isoform is approximately 34 kDa (calculated 33.9 kDa), which is consistent with the observed protein weight in western blotting.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human UPP1, which is highly conserved across human, mouse, and rat, potentially explaining the observed cross-reactivity.
• In Western blot (WB) applications, a single band at approximately 34 kDa is expected; validate band specificity using positive control lysates (e.g., liver, kidney) where UPP1 is expressed.
• For ELISA, this monoclonal antibody can be considered as a capture or detection reagent in sandwich formats; appropriate pairing should be optimized empirically.
• Given the enzyme's role in pyrimidine metabolism, the antibody may be useful in studies of nucleotide synthesis, cancer metabolism, or antiviral drug activation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD-grade antibodies, including this UPP1 rabbit monoclonal antibody, to support diagnostic and research applications in nucleotide metabolism. We provide validated antibody pairs, bulk ancillary reagents, and tailored sourcing solutions to help you accelerate assay development. Contact our team to discuss your custom raw-material needs for metabolic enzyme targets.