Anti-UPK3B Rabbit Polyclonal Antibody for WB, ELISA - Q9BT76

Core Product Specifications and Parameters

Biological Background: Uroplakin-3b Function and Localization

• UPK3B encodes uroplakin-3b, a component of the asymmetric unit membrane (AUM) in terminally differentiated urothelial cells.
• It plays a critical role in mediating AUM-cytoskeleton interactions, essential for maintaining urothelial cell structure.
• UPK3B contributes to the formation of the urothelial glycocalyx, a protective layer that may help prevent bacterial adherence.
• The protein is a transmembrane glycoprotein located at the cell membrane, with a predicted signal peptide and one transmembrane helix.
• UPK3B has multiple aliases including UPIIIb, p35, UP3B, and UPK3B, and is subject to alternative splicing.

Experimental Guidance and Technical Tips

• For western blot, expect a band around 34 kDa; use whole cell lysates from human urothelial cell lines (e.g., T24, RT4) and consider blocking peptide or knockout controls to confirm specificity.
• In ELISA, recombinant protein corresponding to the immunogen region can serve as a positive control; optimize coating concentration and detection antibody conditions.
• As a polyclonal antibody, lot-to-lot variability may occur; validate each new batch in the intended application with appropriate human positive and negative samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to high-quality IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-UPK3B antibody. Our supply chain expertise ensures reliable delivery of bulk ancillary reagents and rare target raw materials, supporting urothelial biology and bladder cancer diagnostic development from concept to clinic.