Anti-UPK3A Rabbit Polyclonal Antibody for WB, IF-P, ELISA - O75631

Core Product Specifications and Parameters

Biological Background: UPK3A Function and Localization

• Uroplakin-3a (UPK3A), also known as UPIII, UP3A, UPK3, and UPIIIA, is a component of the asymmetric unit membrane (AUM) in terminally differentiated urothelial cells.
• It plays a critical role in AUM-cytoskeleton interactions, helping to maintain the unique permeability barrier of the urothelium.
• Contributes to the formation of the urothelial glycocalyx, which is essential for preventing bacterial adherence to the bladder surface.
• Localizes to the endoplasmic reticulum membrane.
• Expressed specifically in the ureter, as indicated by tissue-specific profiling.
• Undergoes alternative splicing, producing isoforms that may have distinct functions or localizations.
• Is a glycoprotein with a single transmembrane helix, consistent with its role as a membrane component.
• The observed protein weight is approximately 31 kDa, in close agreement with the predicted molecular weight of 30.7 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal extracellular domain (amino acids 19–207) of human UPK3A, which may provide good target specificity in immunoassays.
• For Western blotting, a band near 31 kDa is expected; consider validating the signal in your relevant sample system with appropriate positive and negative controls.
• This antibody may be suitable for immunofluorescence on paraffin‑embedded tissue sections (IF‑P) to visualize urothelial cell differentiation and AUM structure.
• ELISA can be employed for quantitative detection of UPK3A in biological fluids or cell lysates; optimize coating and detection conditions for your matrix.
• Given cross‑reactivity with mouse and rat, this antibody is a candidate for studies in rodent models of urinary tract biology; verify dilution and blocking parameters experimentally.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies, recombinant proteins, and ancillary reagents for urological and cell differentiation research. This UPK3A antibody supports studies in urothelial biology and urinary tract infection prevention, and CamelBio can source complementary raw materials such as antibody pairs, ELISA kits, and blocking buffers to accelerate your assay development.