Anti-UPK2 Rabbit Polyclonal Antibody for WB, ELISA - O00526

Core Product Specifications and Parameters

Biological Background: Uroplakin-2 (UPK2) Function and Localization

• Uroplakin-2 (UPK2), also known as Uroplakin II (UPII), is a key protein component of the asymmetric unit membrane (AUM) in terminally differentiated urothelial cells.
• It plays a crucial role in regulating the assembly of the AUM, contributing to the barrier function of the urothelium.
• UPK2 localizes to the cell membrane, where it forms part of the plaque-like structures characteristic of the AUM.
• The protein is specifically expressed in the ureter, highlighting its tissue-restricted distribution.
• Encoded by the UPK2 gene, the protein is synthesized as a precursor with a signal peptide and contains a transmembrane helix, classifying it as a transmembrane glycoprotein.
• Post-translational modifications include cleavage on pair of basic residues and N-glycosylation, which are important for its maturation and function.
• The mature protein has a molecular weight of approximately 19.4 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 85-155 of human UPK2 (extracellular region), so it may recognize epitopes in both native and denatured forms; consider testing in relevant assays.
• For Western blotting, a band around 19 kDa is expected; optimize gel electrophoresis and transfer conditions for low molecular weight proteins.
• Validate antibody specificity using ureter tissue lysates or urothelial cell lines known to express UPK2.
• In ELISA, the recombinant immunogen or purified AUM fractions can serve as coating antigens; include appropriate controls.
• The antibody is provided as a rabbit polyclonal, which may offer robust signal; however, consistency should be monitored between batches.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials. From this anti-UPK2 polyclonal antibody to validated antibody pairs, recombinant uroplakin proteins, and bulk ancillary reagents, we streamline procurement for urothelial biomarker projects. Reach out for tailored sourcing support and technical consultation.