Anti-UPK1B Polyclonal Antibody for Western Blot, ELISA - O75841

Core Product Specifications and Parameters

Biological Background: Uroplakin-1b Function and Localization

• Uroplakin-1b (UPK1B), also known as Tetraspanin-20 and Uroplakin Ib, is a component of the asymmetric unit membrane (AUM) in terminally differentiated urothelial cells.
• It plays an important role in normal bladder epithelial physiology, possibly regulating membrane permeability of superficial umbrella cells and stabilizing the apical membrane through AUM/cytoskeletal interactions.
• UPK1B is a member of the tetraspanin family (TSPAN20), characterized by four transmembrane domains and involvement in cell adhesion, migration, and signaling.
• It is specifically expressed in the bladder epithelium, highlighting its role in urothelial specialization.
• Subcellularly localized to the plasma membrane, UPK1B contributes to the formation of urothelial plaques.
• The protein is glycosylated and possesses transmembrane helices, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region of human UPK1B, which is conserved across species; consider sequence alignment to confirm cross-reactivity with mouse samples.
• For Western blot, the predicted molecular weight is approximately 30 kDa, but endogenous protein may exhibit glycosylation-dependent shifts; validate in your sample system.
• ELISA protocols can be developed using the recombinant immunogen as a positive control.
• Because UPK1B is specific to bladder epithelium, tissue-specific expression should be considered when selecting positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibodies and recombinant proteins. This anti-UPK1B polyclonal antibody supports research into bladder epithelial biology and related disorders, and can be integrated into diagnostic assays. For bulk or customized antibody sourcing needs, reach out to CamelBio.