Anti-UPF2 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q9HAU5

Core Product Specifications and Parameters

Biological Background: UPF2 Function and Localization

• UPF2, also known as Regulator of nonsense transcripts 2 or Up-frameshift suppressor 2 homolog (hUpf2), is a key factor in the nonsense-mediated mRNA decay (NMD) surveillance pathway.
• It is recruited by UPF3B at the cytoplasmic side of the nuclear envelope to the nuclear exon junction complex (EJC) and forms the UPF1-UPF2-UPF3 surveillance complex.
• UPF2 stimulates the ATPase and RNA helicase activities of UPF1, facilitating the degradation of mRNAs containing premature stop codons.
• It binds spliced mRNA and plays a critical role in mRNA quality control.
• UPF2 localizes predominantly to the cytoplasm, particularly in the perinuclear region, consistent with its role in NMD at the ribosome.
• The protein is ubiquitously expressed across tissues, reflecting its fundamental role in gene expression regulation.
• Key post-translational modifications include phosphorylation, and structural features include coiled-coil domains and repeat regions for protein-protein interactions.
• Belongs to a family of RNA-binding proteins involved in mRNA metabolism, with potential implications in cellular stress responses and disease.

Experimental Guidance and Technical Tips

• The immunogen comprises a C-terminal region (amino acids 1153-1272) of human UPF2, which is conserved in mouse, supporting the reported cross-reactivity.
• For western blotting, a band near the theoretical molecular weight of ~148 kDa is expected; consider using a high-molecular-weight marker and appropriate gel percentage for large proteins.
• When performing immunoprecipitation, optimize antibody-to-lysate ratios and consider the size of UPF2 for detection.
• For immunohistochemistry (paraffin-embedded sections), antigen retrieval conditions may need optimization due to the large protein size and nuclear envelope-associated localization.
• ELISA performance may be evaluated with recombinant UPF2 protein fragments or cell lysates; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop sourcing for IVD raw materials, supporting diagnostic manufacturers from concept to clinic. This anti-UPF2 polyclonal antibody is complemented by our provision of validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for NMD pathway research. We facilitate access to rare-target raw materials and technical consulting to accelerate your assay development.