Anti-UPF2 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q9HAU5

Core Product Specifications and Parameters

Biological Background: UPF2 Function and Localization

• UPF2 (Regulator of nonsense transcripts 2), also known as up-frameshift suppressor 2 homolog (hUpf2), is encoded by the UPF2 gene (KIAA1408, RENT2) and is a core component of the nonsense-mediated mRNA decay (NMD) pathway.
• UPF2 is recruited to the exon junction complex (EJC) via UPF3B at the cytoplasmic side of the nuclear envelope, leading to the assembly of the UPF1–UPF2–UPF3 surveillance complex that triggers NMD of mRNAs containing premature stop codons.
• In cooperation with UPF3B, UPF2 stimulates both ATPase and RNA helicase activities of UPF1, thereby facilitating the degradation of aberrant transcripts.
• UPF2 binds spliced mRNA and is essential for the recognition and elimination of nonsense-containing mRNAs to maintain cellular RNA quality control.
• Subcellular localization studies indicate UPF2 resides predominantly in the cytoplasm with a perinuclear enrichment, consistent with its role at the nuclear–cytoplasmic interface during pioneer rounds of translation.
• UPF2 is ubiquitously expressed across human tissues, reflecting its fundamental housekeeping role in NMD.
• UniProt keywords include 3D-structure, Coiled coil, Nonsense-mediated mRNA decay, Phosphoprotein, and RNA-binding, highlighting structural features and regulatory modifications relevant to its function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 1153–1272) of human UPF2; this region may be conserved across species, supporting the observed cross-reactivity with rat.
• For Western blotting, a band at approximately 148 kDa is expected; ensure appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunoprecipitation (IP), the antibody may be suitable for capturing endogenous UPF2 complexes; validate compatibility with downstream assays (e.g., RNA detection, western blot).
• For immunohistochemistry (IHC-P), antigen retrieval and tissue fixation conditions should be optimized for the specific sample type.
• ELISA applications may require determining optimal coating concentrations and detection systems; consider the polyclonal nature for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our platform supports researchers and diagnostic developers working on mRNA surveillance and NMD pathways, with reliable supply capabilities for rare-target raw materials. For your UPF2-related projects, CamelBio streamlines procurement from concept to clinic.