Anti-UPF1/RENT1 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - Q92900

Core Product Specifications and Parameters

Biological Background: UPF1/RENT1 Function and Localization

• UPF1 (Regulator of nonsense transcripts 1), also known as RENT1, NORF1, hUpf1, is an ATP-dependent RNA helicase essential for nonsense-mediated mRNA decay (NMD), a surveillance pathway that degrades aberrant mRNAs with premature stop codons and also modulates expression of normal mRNAs.

• The protein is recruited to translating ribosomes upon termination, where it forms the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, this complex associates with the exon junction complex via UPF2, forming the UPF1-UPF2-UPF3 surveillance complex that triggers mRNA degradation.
  Related references:
  PMID:19417104
  PMID:21419344

• UPF1 undergoes a critical phosphorylation-dephosphorylation cycle. Its phosphorylation by SMG1 kinase is a key step in NMD, and dephosphorylation by PP2A enables recycling of NMD factors.
  Related references:
  PMID:11544179
  PMID:25220460

• UPF1 can also activate NMD independently of UPF2 and UPF3, indicating alternative NMD pathways.
  Related references:
  PMID:18447585

• Beyond NMD, UPF1 plays a role in replication-dependent histone mRNA degradation at the end of S phase, a function independent of UPF2.
  Related references:
  PMID:16086026
  PMID:18172165

• UPF1 possesses ATP-dependent 5'-3' helicase activity on RNA:DNA, RNA:RNA, and DNA:DNA substrates. It also has ssDNA-stimulated ATPase activity and binds RNA; ATP addition decreases RNA binding.
  Related references:
  PMID:10999600
  PMID:18066079
  PMID:26138914

• Subcellularly, UPF1 is primarily cytoplasmic, but also localizes to P-bodies, the perinuclear region, and the nucleus.

• Keywords associated with UPF1 include Helicase, ATP-binding, Zinc-finger, Phosphoprotein, Nonsense-mediated mRNA decay, and Host-virus interaction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human UPF1; this antibody may recognize full-length UPF1 and any isoforms sharing this N-terminal sequence.
• Predicted molecular weight is 124 kDa; for Western blotting, consider using an appropriate gel percentage (e.g., 8% SDS-PAGE) and sufficient transfer time for high molecular weight proteins.
• This rabbit monoclonal antibody offers high specificity and low background; it has been validated for WB, IF/ICC, IP, and ELISA. For each application, titrate the antibody and validate in the relevant sample system.
• Cross-reactivity with mouse and rat is reported, making it suitable for studies in these model organisms; however, endogenous expression levels may vary across tissues and cell lines.
• For IF/ICC, incorporate appropriate fixation and permeabilization steps, and use the antibody at a dilution optimized for your specific cellular context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw material sourcing, supporting diagnostic manufacturers and research labs from concept to clinic. For NMD and RNA biology research, CamelBio provides access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, along with rare target raw-material sourcing to accelerate your assay development and production.