Anti-UPF1/RENT1 Polyclonal Antibody for WB, IHC-P, ELISA - Q92900

Core Product Specifications and Parameters

Biological Background: UPF1 Function and Localization

• UPF1/RENT1 is an ATP-dependent RNA helicase and central factor in nonsense-mediated mRNA decay (NMD), targeting transcripts with premature termination codons. Related references: PMID:11163187 PMID:16086026 PMID:18172165
• It catalyzes 5'-3' unwinding of RNA:DNA, RNA:RNA, and DNA:DNA substrates with a 5' extension. Related references: PMID:10999600 PMID:18066079 PMID:21419344 PMID:23275559 PMID:26138914 PMID:30218034
• Phosphorylation of UPF1 by SMG1 kinase is a critical regulatory step in NMD, while dephosphorylation by PP2A recycles NMD factors. Related references: PMID:11544179 PMID:12554878 PMID:25220460
• UPF1 is recruited to stalled ribosomes via release factors, forming the SURF complex, and interacts with the exon junction complex (EJC) through UPF2 and UPF3 to assemble the surveillance complex. Related references: PMID:19417104 PMID:21419344
• UPF1 also mediates replication-dependent histone mRNA degradation at the end of S phase and can activate NMD independently of UPF2 or the EJC. Related references: PMID:18447585 PMID:16086026 PMID:18172165
• Subcellularly, UPF1 localizes predominantly to the cytoplasm, P-bodies, and perinuclear region, with some nuclear presence.
• It is ubiquitously expressed and contains zinc finger motifs, ATP-binding, and RNA-binding domains.

Experimental Guidance and Technical Tips

• For Western blotting, use whole cell lysates from human or mouse cell lines; the expected band is at ~124 kDa.
• For IHC-P, test antigen retrieval conditions on formalin-fixed paraffin-embedded sections.
• The immunogen (aa 270-370) lies in an internal region; the antibody may detect both phosphorylated and unphosphorylated forms; consider phosphatase treatment to assess phospho-specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies high-quality IVD raw materials including this validated anti-UPF1 polyclonal antibody for use in NMD research and diagnostic development. As a one-stop sourcing partner, we offer customized antibody pairs, bulk reagents, and rare target raw-material procurement to streamline your assay development from concept to clinic. Explore our comprehensive portfolio for all your IVD needs.