Anti-UNC5B Polyclonal Antibody for WB, ELISA - Q8IZJ1

Core Product Specifications and Parameters

Biological Background: UNC5B Function and Localization

• Protein identity: Netrin receptor UNC5B (also known as protein unc-5 homolog 2, protein unc-5 homolog B, or p53-regulated receptor for death and life protein 1/p53RDL1) is a single-pass transmembrane receptor that belongs to the UNC5 netrin receptor family.
• Core function in axon guidance: UNC5B functions as a receptor for netrin, mediating axon repulsion of neuronal growth cones during development of the nervous system. This repulsive signaling may occur through association with DCC.
• Role in angiogenesis: Negatively regulates vascular branching by mediating retraction of tip cell filopodia on endothelial growth cones in response to netrin, thereby controlling blood vessel formation.
• Dependence receptor activity: Acts as a dependence receptor that triggers apoptosis in the absence of netrin ligand. UNC5B activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308, increasing catalytic activity and promoting cell death. Related references: PMID:12598906
• Subcellular localization: Localizes to the cell membrane and is enriched in membrane rafts, consistent with its role in signal transduction at the cell surface.
• Post‑translational modifications: UNC5B is subject to alternative splicing, disulfide bond formation, glycosylation, palmitoylation, and phosphorylation, which may regulate its stability, localization, and activity.
• Tissue distribution: Highly expressed in brain; also detected at lower levels in developing lung, cartilage, kidney, and hematopoietic/immune tissues.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 290–377 within the extracellular domain of human UNC5B. This region may be well‑exposed in native conformations, so consider its utility for detecting the full‑length protein (~104 kDa) in WB.
• For ELISA applications, the recombinant immunogen itself or purified UNC5B protein can serve as a positive control to validate assay sensitivity and specificity.
• Cross‑reactivity is reported for human, mouse, and rat; however, sequence variations between species within the immunogen region should be verified, and detection in each species should be empirically validated in the relevant sample system.
• Because UNC5B is a transmembrane receptor enriched in membrane rafts, adequate membrane solubilization (e.g., using non‑denaturing detergents) may improve detection efficiency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For your UNC5B‑related research in neurodevelopment, angiogenesis, or apoptosis, we can supply validated antibody pairs, optimized polyclonal antibodies like this anti‑UNC5B product, bulk ancillary reagents, and even assist in rare target raw‑material sourcing. Let CamelBio bridge the gap between your research needs and reliable procurement.