Anti-UNC13B Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O14795

Core Product Specifications and Parameters

Biological Background: UNC13B Function and Localization

• Protein unc-13 homolog B (UNC13B), also known as Munc13-2, is a member of the UNC13 family essential for synaptic vesicle priming and neurotransmitter release.
• Functions as a key effector of the diacylglycerol second messenger pathway, playing a critical role in vesicle maturation during exocytosis (By similarity).
• Facilitates the priming of synaptic vesicles prior to fusion and contributes to the activity-dependent refilling of the readily releasable vesicle pool (RRP) (By similarity).
• Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic synapses, but not inhibitory/GABA-mediated synapses (By similarity).
• In cooperation with UNC13A, controls the location and efficiency of neuronal dense core vesicle fusion and synaptic release (By similarity).
• Localized to the cytoplasm, cell membrane, and synapse, reflecting its role in membrane-associated exocytotic processes.
• Expressed in kidney cortical epithelial cells and brain, highlighting its tissue-specific functions.
• Undergoes phosphorylation and contains zinc finger domains, indicating regulation by post-translational modifications and metal binding.

Experimental Guidance and Technical Tips

• The antibody was generated against an N-terminal fragment (amino acids 1–350) of human UNC13B; verify specificity using appropriate controls.
• For Western blotting, the predicted molecular weight is 181 kDa; use a suitable gel percentage (e.g., 6%–8% SDS-PAGE) and optimized transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), test the antibody on known positive tissues or cell lines such as brain or kidney sections.
• Cross-reacts with mouse UNC13B; murine samples can serve as an additional experimental model.
• As a polyclonal antibody, lot-to-lot consistency may vary; include positive and negative controls in each experiment to ensure reproducibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including validated antibodies, antigens, and ancillary reagents. For neuroscience and exocytosis studies targeting UNC13B, we offer reliable polyclonal antibodies alongside sourcing services for rare targets and bulk orders. From concept to clinic, CamelBio supports your assay development with one-stop procurement solutions.