Anti-UMPS Polyclonal Antibody for WB, IF/ICC, ELISA - P11172

Core Product Specifications and Parameters

Biological Background: UMPS Function and Localization

• UMPS (uridine monophosphate synthase) is a bifunctional enzyme that catalyzes the final two steps of de novo pyrimidine biosynthesis.
• The N-terminal domain possesses orotate phosphoribosyltransferase (OPRT) activity, converting orotate to orotidine-5'-monophosphate (OMP).
• The C-terminal domain contains orotidine-5'-monophosphate decarboxylase (ODC) activity, decarboxylating OMP to uridine monophosphate (UMP).
• Mutations in UMPS cause hereditary orotic aciduria, a rare disorder of pyrimidine metabolism.
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzymatic function or stability.
• Alternative splicing generates multiple isoforms with potentially distinct tissue-specific roles.
• UniProt keywords highlight it as a multifunctional enzyme, decarboxylase, glycosyltransferase, transferase, lyase, and phosphoprotein.
• The calculated molecular weight is approximately 52.2 kDa, consistent with the observed band at ~52 kDa.

Experimental Guidance and Technical Tips

• The immunogen maps to amino acids 100–260 within the OPRT domain, so the antibody is expected to recognize both the OPRT domain and full-length UMPS.
• For Western blot, a band near 52 kDa is anticipated; validate with appropriate positive and negative controls in your sample system.
• This polyclonal antibody has been tested in WB, IF/ICC, and ELISA; consider optimizing dilutions empirically for your specific experimental conditions.
• Reported cross-reactivity with human, mouse, and rat makes it suitable for comparative studies across these species.
• For immunohistochemical applications, confirm antigen retrieval compatibility and antibody specificity in the tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and researchers with a comprehensive portfolio of IVD raw materials. This anti-UMPS polyclonal antibody supports research into pyrimidine biosynthesis and related disorders, complementing our offering of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Our integrated supply solutions streamline your workflow from concept to clinic.