Anti-UMPS Polyclonal Antibody for WB, ELISA - P11172

Core Product Specifications and Parameters

Biological Background: UMPS Function and Localization

• UMPS (Uridine 5'-monophosphate synthase) is a bifunctional enzyme that catalyzes the final two steps of de novo pyrimidine biosynthesis: orotate phosphoribosyltransferase (OPRT) converts orotate to OMP, and orotidine-5'-phosphate decarboxylase (ODC) then converts OMP to UMP.
• The protein is also known as OPRT and ODCase, reflecting its dual enzymatic activities.
• As a critical enzyme in pyrimidine metabolism, UMPS ensures the supply of UMP for RNA and DNA synthesis, as well as for other cellular processes.
• The UMPS gene undergoes alternative splicing, generating multiple transcript variants that may influence enzyme function or regulation.
• Mutations in UMPS are associated with hereditary orotic aciduria, a rare metabolic disorder characterized by megaloblastic anemia and orotic acid crystalluria (keyword: Disease variant).
• Post-translational modifications of UMPS include acetylation and phosphorylation, which may regulate its activity or stability (keywords: Acetylation, Phosphoprotein).
• The three-dimensional structure of UMPS has been determined, revealing the spatial organization of its OPRT and ODC domains (keyword: 3D-structure).

Experimental Guidance and Technical Tips

• The immunogen (amino acids 181-480) encompasses a region that contains functional domains; therefore, the antibody may detect multiple splice variants containing this sequence.
• For Western blotting, consider using whole-cell lysates from human cell lines known to express UMPS (e.g., HeLa or HEK293) and include appropriate loading controls.
• In ELISA, determine the optimal coating antigen and antibody concentration through checkerboard titration, and confirm specificity using both positive and negative control antigens.
• Since the antibody is validated for human reactivity, cross-reactivity with other species cannot be assumed and should be tested individually.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate your diagnostic development. This UMPS antibody is suitable for research into pyrimidine metabolism and related disorders, and CamelBio can support your project from concept to clinic with rare target raw-material sourcing and technical consultation.