Anti-ULK4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96C45

Core Product Specifications and Parameters

Biological Background: ULK4 Function and Localization

• ULK4 is a pseudokinase belonging to the Unc-51-like kinase family, also known as FAM7C1 and REC01035, and is characterized by a lack of catalytic kinase activity, functioning instead as a molecular adapter.
• In the hedgehog signaling pathway, ULK4 acts at the primary cilium to mediate the association between STK36 kinase and the GLI2 transcription factor, promoting GLI2 phosphorylation and subsequent release from SUFU inhibition, enabling GLI2 nuclear translocation and target gene activation. Related references: PMID:38096226
• ULK4 is localized to cell projections, specifically the cilium, consistent with its role in ciliary hedgehog signal transduction.
• At the protein level, ULK4 expression is predominantly detected in the brain, particularly in postmitotic neurons including GABAergic neurons, and is absent in astrocytes.
• The protein has an ATP-binding domain and nucleotide-binding capacity, though its pseudokinase classification implies non-canonical nucleotide utilization.
• It contains multiple repeat regions and is subject to phosphorylation, as indicated by proteomics identification, suggesting regulatory post-translational modifications.
• ULK4’s adapter function in GLI2 activation is critical for proper hedgehog signaling, which governs embryonic development, stem cell maintenance, and tissue regeneration.

Experimental Guidance and Technical Tips

• For Western blotting, given the high molecular weight of ULK4 (approximately 142 kDa), consider using a low-percentage polyacrylamide gel (e.g., 6-8%) with extended transfer times to ensure efficient protein transfer.
• In immunofluorescence or immunocytochemistry, because ULK4 localizes to cilia, optimize fixation and permeabilization conditions to preserve ciliary structures, and confirm co-localization with ciliary markers such as acetylated tubulin or Arl13b.
• Validate the antibody specificity in your sample system by including known ULK4-expressing cell lines or tissues (e.g., neuronal cultures or brain sections) and performing knockdown/knockout controls where feasible.
• As a polyclonal antibody, lot-to-lot consistency may vary; we recommend titrating each new lot and including appropriate positive and negative controls in every experiment.
• For ELISA applications, ensure the recombinant immunogen or full-length ULK4 protein is used for coating to confirm reactivity and determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, connecting diagnostic developers and researchers with high-quality antibodies like this anti-ULK4 polyclonal antibody, ideal for neuroscience and cell signaling investigations. Leveraging a broad network of validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents, we support your entire project—from initial concept to clinical application. Trust CamelBio to streamline your procurement of rare target reagents, including pseudokinases and ciliary proteins, with reliable supply and expert guidance.