Anti-ULK3 Monoclonal Antibody for WB, IF/ICC, ELISA - Q6PHR2

Core Product Specifications and Parameters

Biological Background: ULK3 Function and Localization

• ULK3 (Unc-51-like kinase 3) is a serine/threonine protein kinase that acts as a key regulator of smoothened signaling in the hedgehog pathway and autophagy initiation. Related references: PMID:19279323 PMID:19878745 PMID:20643644 PMID:31279575

• In the presence of hedgehog ligands (DHH, IHH, SHH), ULK3 autophosphorylates and phosphorylates GLI2 (and to a lesser extent GLI1 and GLI3), promoting their dissociation from SUFU, nuclear translocation, and transcriptional activation. Related references: PMID:19878745 PMID:20643644 PMID:31279575

• In the absence of hedgehog ligand, ULK3 interacts with SUFU, which inactivates its kinase activity and prevents GLI protein phosphorylation, ensuring pathway repression. Related references: PMID:20643644

• Beyond hedgehog signaling, ULK3 induces autophagy in response to cellular senescence, linking it to cellular quality control and aging. Related references: PMID:19279323

• ULK3 is primarily localized in the cytoplasm and is widely expressed across tissues, with highest levels in fetal brain and adult brain, liver, and kidney; within the nervous system, it is particularly abundant in the hippocampus.

• The protein contains kinase domains, ATP-binding sites, and undergoes alternative splicing; it is classified under the serine/threonine-protein kinase family and is a known phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 373–472 of human ULK3, a region within the kinase domain. Researchers may consider this epitope location when designing complementary detection reagents.
• This antibody has been tested in WB, IF/ICC, and ELISA. For immunoblotting, the expected band is approximately 53 kDa; potential isoforms from alternative splicing may appear at different sizes and should be validated in the relevant sample system.
• Taking into account the cross-reactivity with both human and mouse, a pilot experiment with appropriate positive and negative controls is recommended to confirm specificity before large-scale studies.
• For IF/ICC applications, cell fixation and permeabilization methods should be carefully optimized, as ULK3 is a cytoplasmic protein.
• Kinase activity and pathway activation can affect ULK3 expression; thus, using samples where hedgehog signaling or autophagy is modulated could aid in assessing antibody performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing a comprehensive range of IVD raw materials, connecting diagnostic manufacturers, laboratories, and research institutes with high-quality reagents. For investigations into kinase signaling, hedgehog pathway, and autophagy, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities also extend to rare target raw materials, ensuring a reliable supply chain from concept to clinic.