Anti-ULK1 Rabbit Polyclonal Antibody for WB and ELISA - O75385

Core Product Specifications and Parameters

Biological Background: ULK1 Function and Localization

• ULK1 is a serine/threonine-protein kinase, also known as autophagy-related protein 1 homolog (ATG1), that plays a central role in autophagy initiation (PubMed:18936157, PubMed:21460634, PubMed:21795849). Related references: PMID:18936157 PMID:21460634 PMID:21795849
• It functions upstream of the phosphatidylinositol 3-kinase complex (PIK3C3) to regulate autophagophore formation, a critical step in autophagosome biogenesis (PubMed:18936157, PubMed:21460634, PubMed:21795849). Related references: PMID:18936157 PMID:21460634 PMID:21795849
• ULK1 participates in regulatory feedback loops with mTORC1, acting both as a downstream effector and a negative regulator via interaction with RPTOR (PubMed:21795849). Related references: PMID:21795849
• It is activated by AMPK-mediated phosphorylation and can reciprocally phosphorylate AMPK subunits (PRKAA1, PRKAB2, PRKAG1) to modulate AMPK activity (PubMed:21460634). Related references: PMID:21460634
• ULK1 phosphorylates multiple autophagy-related substrates, including SESN2, SQSTM1, FLCN, AMBRA1, and ATG4B, to fine-tune autophagic flux (PubMed:25040165, PubMed:37306101, PubMed:25126726, PubMed:20921139, PubMed:28821708). Related references: PMID:25040165 PMID:37306101 PMID:25126726 PMID:20921139 PMID:28821708
• Beyond autophagy, ULK1 plays a role early in neuronal differentiation and is required for granule cell axon formation (PubMed:11146101). Related references: PMID:11146101
• It localizes to the cytoplasm and preautophagosomal structures, consistent with its role in autophagosome initiation.
• Ubiquitously expressed across adult tissues including skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

Experimental Guidance and Technical Tips

• Since the immunogen is a recombinant human ULK1 protein, this polyclonal antibody is expected to recognize multiple epitopes, which may provide strong signal in Western blot (WB) applications. Optimize protein loading and blocking conditions for your specific sample type.
• The antibody cross-reacts with mouse ULK1, making it suitable for studies in mouse models; validate in the relevant mouse cell or tissue lysates to confirm specificity.
• For ELISA, consider using the antibody as a capture or detection reagent after confirming its performance with recombinant ULK1 protein or endogenous target in cell lysates.
• As with any polyclonal antibody, lot-to-lot consistency can vary; always validate a new lot against known positive and negative controls before use in critical assays.
• The predicted molecular weight of ULK1 is ~113 kDa; in WB, band appearance may vary depending on cell type and post-translational modifications; consider using phosphatase inhibitors in lysis buffer to preserve phosphorylation states.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio partners with diagnostic manufacturers and research laboratories to deliver a comprehensive portfolio of IVD raw materials, from validated antibody pairs to bulk ancillary reagents. For autophagy and cell signaling studies, we offer access to optimized monoclonal and polyclonal antibodies against key targets like ULK1. With our one-stop sourcing model, we support your projects from concept to clinic, including rare target sourcing and technical consultation.