Anti-ULK1 Polyclonal Antibody for WB, IF/ICC, ELISA - O75385

Core Product Specifications and Parameters

Biological Background: ULK1 Function and Localization

• Serine/threonine-protein kinase ULK1, the mammalian homolog of yeast ATG1, is a central initiator of autophagy. It acts upstream of the class III PI3K complex (PIK3C3) to regulate autophagophore formation and participates in regulatory feedback loops with mTORC1 and AMPK. Related references: PMID:18936157 PMID:21460634 PMID:21795849
• Phosphorylates multiple autophagy-related substrates, including ATG13, RPTOR, SESN2, SQSTM1/p62, FLCN, AMBRA1, and ATG4B, thereby coordinating autophagosome initiation, nucleation, and maturation. Related references: PMID:18936157 PMID:25040165 PMID:37306101 PMID:25126726 PMID:20921139 PMID:28821708
• Plays a role early in neuronal differentiation and is required for granule cell axon formation. Related references: PMID:11146101
• Ubiquitously expressed across adult human tissues, with detection in skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
• Localizes predominantly to the cytoplasm and cytosol, and is recruited to preautophagosomal structures upon autophagy induction.
• Post-translationally modified by acetylation and ubiquitin-like conjugation; a phosphoprotein with characterized kinase function.

Experimental Guidance and Technical Tips

• For Western blotting, a band at approximately 113 kDa is expected. Optimized lysis buffers and thorough homogenization may improve detection of this large protein.
• When performing immunofluorescence/immunocytochemistry, permeabilization conditions should be validated to allow antibody access to cytoplasmic and preautophagosomal pools.
• The polyclonal nature may confer robust signal but should be validated in the relevant species (human, mouse, rat) and experimental system to confirm specificity.
• For ELISA applications, the peptide immunogen suggests suitability for peptide-based assays; consider appropriate coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this anti-ULK1 polyclonal antibody as part of a comprehensive catalog of IVD raw materials for autophagy and cell signaling research. With validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, we support diagnostic manufacturers in streamlining procurement from concept to clinic. For specialized needs, including rare target sourcing, our team provides reliable access to critical raw materials.