Anti-ULK1 (phospho S555) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - O75385

Core Product Specifications and Parameters

Biological Background: ULK1 Function and Localization

• Serine/threonine-protein kinase ULK1 (Unc-51-like kinase 1, ATG1) is a key initiator of autophagy, particularly in response to starvation. Related references: PMID:18936157, PMID:21460634, PMID:21795849
• Acts upstream of the PI3K complex to regulate autophagophore formation, a critical step in autophagosome biogenesis. Related references: PMID:18936157, PMID:21460634, PMID:21795849
• Functions within regulatory feedback loops: it is a downstream effector of mTORC1 and also negatively regulates mTORC1 activity via interaction with RPTOR. Related references: PMID:21795849
• Activated via AMPK-mediated phosphorylation and, in turn, phosphorylates AMPK subunits to modulate AMPK signaling. Related references: PMID:21460634
• Localizes to the cytosol and preautophagosomal structures, consistent with its role in early autophagosome formation.
• Ubiquitously expressed in adult tissues including skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
• Involved in neuronal differentiation and axon formation, indicating roles beyond canonical autophagy. Related references: PMID:11146101
• Subject to multiple post-translational modifications: phosphorylation, acetylation, and ubiquitination, which regulate its activity and interactions.

Experimental Guidance and Technical Tips

• This antibody is raised against a phosphorylated peptide, so it specifically recognizes ULK1 phosphorylated at Ser555. Consider using phosphatase inhibitors in lysis buffers to preserve phosphorylation.
• For WB, validate specificity by comparing untreated and phosphatase-treated samples or using non-phospho peptide competition.
• IF/ICC may require permeabilization and fixation conditions that preserve phospho-epitopes; consider testing methanol fixation.
• For ELISA, ensure coating antigen presents the phosphorylated epitope; use an unphosphorylated control to confirm specificity.
• Cross-reacts with human and mouse; validate reactivity in your species of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your autophagy research with this phospho-specific ULK1 rabbit monoclonal antibody and a comprehensive portfolio of IVD raw materials. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing, we provide everything needed to move from concept to clinic. Let us be your reliable partner in accelerating diagnostic development.