Anti-ULBP2 Rabbit Monoclonal Antibody for WB and ELISA - Q9BZM5

Core Product Specifications and Parameters

Biological Background: ULBP2 Function and Localization

• UL16-binding protein 2 (ULBP2), also known as ALCAN-alpha, N2DL2, NKG2DL2, or RAET1H, is a member of the ULBP/RAET1 family of MHC class I-related molecules.
• ULBP2 functions as a ligand for the KLRK1/NKG2D receptor, an activating receptor expressed on natural killer (NK) cells, CD8+ T cells, and γδ T cells.
• Binding of ULBP2 to NKG2D triggers NK cell-mediated cytotoxicity, playing a critical role in immune surveillance against tumors and infected cells.
• ULBP2 is a GPI-anchored glycoprotein expressed on the cell membrane; it can also be found in the endoplasmic reticulum and as a secreted form.
• The protein contains a signal peptide, an extracellular region with MHC class I-like domains, and a C-terminal GPI-anchor attachment site; it has a predicted molecular weight of approximately 27 kDa.
• ULBP2 is preferentially expressed in various cancer cell lines and in fetal tissues, but is generally absent in normal adult tissues, making it an attractive target for cancer immunotherapy.
• ULBP2 may also be involved in host-virus interactions, as some viruses encode proteins that interfere with NKG2D ligand expression to evade immune detection.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a recombinant protein encompassing amino acids 26-217 of human ULBP2, covering the majority of its extracellular domain.
• For Western blot analysis, a band corresponding to full-length ULBP2 at ~27 kDa may be observed; post-translational modifications such as glycosylation could result in a slightly higher apparent molecular weight.
• When performing ELISA, consider using recombinant ULBP2 as a positive control and proper blocking agents to minimize nonspecific binding.
• It is recommended to validate antibody performance in the intended sample system (e.g., cancer cell lines or transfected cells) under your specific experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research laboratories, and academic institutes a streamlined channel for procuring high-quality IVD raw materials. With this anti-ULBP2 monoclonal antibody, we support research into NK cell biology, cancer immunology, and therapeutic target development. Beyond individual reagents, CamelBio can supply validated antibody pairs, bulk quantities of ancillary reagents, and assist in sourcing rare-target raw materials to accelerate your assay development from concept to clinic.