Anti-ULBP1 Rabbit Monoclonal Antibody for WB, ELISA - Q9BZM6

Core Product Specifications and Parameters

Biological Background: ULBP1 Function and Localization

• ULBP1 (UL16-binding protein 1), also known as ALCAN-beta, NKG2D ligand 1 (N2DL-1/NKG2DL1), and retinoic acid early transcript 1I, is a cell-surface glycoprotein belonging to the RAET1 family. It shares structural similarity with MHC class I molecules but does not present peptides.
• The primary function of ULBP1 is to bind and activate the KLRK1/NKG2D receptor on natural killer (NK) cells, thereby triggering NK cell-mediated cytotoxicity and cytokine release, crucial for immune surveillance against infected or transformed cells.
• ULBP1 is a GPI-anchored membrane protein localized mainly at the cell membrane, but it is also detected in the endoplasmic reticulum, reflecting its secretory pathway processing and regulation.
• ULBP1 is expressed in a diverse array of tissues, including immune cells (T-cells, B-cells), erythroleukemia cell lines, and organs such as heart, brain, lung, liver, testis, lymph node, thymus, tonsil, and bone marrow, as well as in fetal tissues (heart, brain, lung, liver), indicating both immune and non-immune roles.
• As a stress-induced ligand, ULBP1 is upregulated in response to DNA damage, viral infection, and cellular transformation, serving as a “danger signal” to eliminate compromised cells. Its expression can be exploited by viruses (e.g., cytomegalovirus) to evade immune detection.
• Key post-translational features include a signal peptide for secretion, a GPI anchor for membrane attachment, N-linked glycosylation, and one or more disulfide bonds, which stabilize its extracellular domain for NKG2D interaction.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein corresponding to the N-terminal region (amino acids 1–100) of human ULBP1 (NP_079494.1). This fragment covers the signal peptide and part of the extracellular domain, which may be suitable for detecting both full-length endogenous and recombinant ULBP1.
• For western blot (WB) applications, consider using whole-cell lysates from ULBP1-expressing cell lines such as K562 (erythroleukemia) or Jurkat (T-cell leukemia). Given the predicted molecular weight of ~28 kDa (core protein; glycosylation may increase apparent mass), optimize reducing conditions and protein loading.
• The antibody also cross-reacts with mouse ULBP1, making it a potential tool for cross-species studies. However, validate reactivity in the relevant mouse tissue or cell models, as epitope conservation may affect performance.
• In ELISA, consider pairing this monoclonal antibody with a polyclonal detection antibody to establish a sandwich assay for soluble ULBP1, which can be shed from the cell surface. Optimize the coating and detection steps for sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research institutions by providing a comprehensive portfolio of IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. For ULBP1-related immune surveillance studies or NKG2D ligand-based assay development, we can source rare-target reagents and offer technical consultation to streamline your transition from research to clinical application. Whether you need custom antibody generation or ready-to-use components, CamelBio bridges the gap between concept and clinic, helping you achieve reliable and scalable assay performance.