Anti-UIMC1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96RL1

Core Product Specifications and Parameters

Biological Background: UIMC1 Function and Localization

• RAP80 is a ubiquitin-binding protein that specifically recognizes and binds Lys-63-linked ubiquitin chains with high affinity, while showing weak binding to monoubiquitin and no affinity for Lys-48-linked chains. Related references: PMID:24627472, PMID:19328070
• Core component of the BRCA1-A complex, directing BRCA1-BARD1 to DNA double-strand breaks via binding to Lys-63-ubiquitinated histones H2A and H2AX.
• The BRCA1-A complex also possesses deubiquitinase activity that specifically removes Lys-63-linked ubiquitin from histones H2A and H2AX at damage sites.
• May function as a transcriptional repressor by inhibiting NR6A1 interaction with NCOR1 corepressor.
• Localizes predominantly to the nucleus, consistent with its role in DNA damage signaling and chromatin regulation.
• Expressed in testis, ovary, thymus, and heart; also detected in testicular germ cells.
• Post-translational modifications include phosphorylation and ubiquitination; contains zinc-finger domains indicative of DNA repair function.
• Alternative splicing produces multiple isoforms, with the canonical isoform having a molecular weight of approximately 80 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 1-150 amino acids of human UIMC1; confirm immunoreactivity in your target species and relevant isoform.
• For Western blot, the predicted molecular weight is ~80 kDa; validate with appropriate positive control lysates from human, mouse, or rat cells.
• IF/ICC applications may require nuclear permeabilization; include DNA damage inducers if studying RAP80 recruitment to foci.
• ELISA can be optimized using recombinant protein or peptide as coating antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, providing validated antibodies, bulk ancillary reagents, and rare-target sourcing to support DNA damage repair research. Our supply network includes optimized polyclonal antibodies like anti-UIMC1, covering multiple applications and species reactivity, ready for diagnostic development. Partner with us to streamline your raw material procurement from discovery to clinic.