Anti-UHRF2 Rabbit Polyclonal Antibody for WB and ELISA - Q7TMI3

Core Product Specifications and Parameters

Biological Background: UHRF2 Function and Localization

• UHRF2 is an E3 ubiquitin-protein ligase containing UBL, PHD, and RING finger domains, and is also known as NIRF, Np97, and UHRF2.
• Acts as a specific 5-hydroxymethylcytosine (5hmC) reader, recruiting substrates for ubiquitination to maintain 5mC levels and regulate neuron-related gene expression. Related references: PMID:23434322, PMID:28402695, PMID:28115522
• Participates in cell cycle control by ubiquitinating cyclins CCND1 and CCNE1, inducing G1 arrest, and targeting PCNP for proteasomal degradation (By similarity).
• In the DNA damage response, it ubiquitinates p21/CDKN1A for degradation and serves as an interstrand cross-link sensor, cooperating with UHRF1 to promote FANCD2 monoubiquitination and repair (By similarity).
• Interacts with ATR upon UV irradiation to enhance ATR activation, contributing to the UV damage response (By similarity).
• Localizes predominantly to the nucleus and chromosomes.
• Detected in multiple tissues including thymus, spleen, lung, adrenal gland, ovary, and brain regions (cerebellum, hippocampus, cerebral cortex).
• Classified by keywords such as alternative splicing, cell cycle, chromosome, DNA-binding, metal/zinc binding, phosphoprotein, transferase, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 76–359, a region conserved across human, mouse, and rat UHRF2, supporting the reported multispecies cross-reactivity.
• For Western blot, consider testing a range of antibody dilutions (e.g., 1:1000–1:5000) and validate with lysates from tissues with known UHRF2 expression (brain, thymus, spleen).
• For ELISA, optimize coating antigen concentration and detection conditions; pairing with a matched detection antibody may enhance sensitivity.
• Ensure proper storage at -20°C or as recommended; avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development by providing validated antibody pairs, high-quality recombinant proteins, and ancillary reagents tailored to diagnostic needs. With this anti-UHRF2 antibody, researchers and manufacturers gain a reliable tool for epigenetics and cell cycle studies. Our end-to-end sourcing services support your transition from concept to clinic efficiently.