Anti-Uhrf2 Rabbit Polyclonal Antibody for WB and ELISA - Q7TMI3

Core Product Specifications and Parameters

Biological Background: Uhrf2 Function and Localization

• Uhrf2 (also known as NIRF, Np95-like ring finger protein, Np97) is an E3 ubiquitin-protein ligase that plays important roles in DNA methylation, histone modifications, cell cycle, and DNA repair.
• Uhrf2 acts as a specific reader for 5-hydroxymethylcytosine (5hmC), recruiting substrates for ubiquitination (PubMed:23434322, PubMed:28402695), which maintains 5mC levels at specific genomic loci and regulates neuron-related gene expression (PubMed:28115522). Related references: PMID:23434322 PMID:28402695 PMID:28115522
• In cell cycle regulation, Uhrf2 ubiquitinates cyclins CCND1 and CCNE1, inducing G1 arrest, and targets PCNP for proteasomal degradation.
• It contributes to DNA damage repair by ubiquitinating p21/CDKN1A, acting as an interstrand cross-link sensor, and cooperating with UHRF1 to promote FANCD2 monoubiquitination and ATR activation (By similarity).
• Uhrf2 localizes to the nucleus and chromosomes.
• Uhrf2 is mostly detected in thymus, spleen, lung, adrenal gland, ovary, and brain regions including cerebellum, hippocampus, and cerebral cortex.
• Keywords associated with Uhrf2: Alternative splicing, phosphoprotein, zinc-finger, Ubl conjugation, cell cycle, chromosome, DNA-binding.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein spanning amino acids 76-359 of mouse Uhrf2. Researchers may consider this region for designing their own constructs.
• This antibody is specified for Western blot (WB) and ELISA. Users are advised to validate in the relevant sample system, considering that endogenous protein weight is approximately 90 kDa.
• Cross-reactivity with mouse and rat suggests suitability for rodent studies. For other species, verification is recommended.
• When performing WB, consider using whole cell lysates or nuclear extracts from mouse or rat tissues such as thymus, spleen, or brain, where Uhrf2 is highly expressed.
• For ELISA, the antibody may be used to capture or detect recombinant Uhrf2; optimization of coating and detection conditions is recommended.
• Given Uhrf2's role in ubiquitination, researchers may pair this antibody with ubiquitin-specific probes or inhibitors to study Uhrf2-mediated pathways.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of high-quality IVD raw materials, enabling diagnostic manufacturers and researchers to accelerate assay development from concept to clinical application. This anti-Uhrf2 antibody belongs to our range of reagents for epigenetic and cell cycle research, supporting studies in DNA methylation and protein ubiquitination. Whether you need validated antibody pairs, recombinant proteins, or custom raw-material sourcing, CamelBio serves as your reliable supply partner.