Anti-UHRF1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q96T88

Core Product Specifications and Parameters

Biological Background: UHRF1 Function and Localization

• UHRF1 (UniProt Q96T88), also known as inverted CCAAT box-binding protein of 90 kDa (ICBP90), nuclear protein 95 (Np95), RING finger protein 106, or hUHRF1, is a multidomain E3 ubiquitin-protein ligase containing zinc finger motifs, a PHD finger, and a RING finger.
• Functions as a key epigenetic regulator that bridges DNA methylation and chromatin modification. Related references: PMID:10646863 PMID:15009091 PMID:19056828
• Plays a critical role in DNA methylation inheritance by promoting the recruitment of DNA methyltransferase 1 (DNMT1) to hemimethylated DNA during replication. Related references: PMID:23022729 PMID:24013172 PMID:27595565
• Acts as a histone reader and writer: specifically recognizes histone H3 trimethylated at Lys-9 and unmethylated at Arg-2 (H3K9me3 and H3R2me0), then catalyzes monoubiquitination of H3 at Lys-18 (and to a lesser extent Lys-14 and Lys-23) to create a docking site for DNMT1. Related references: PMID:15361834 PMID:17673620 PMID:17967883 PMID:27595565 PMID:24013172
• Enriched in pericentric heterochromatin and also localizes to euchromatic regions, where it recruits chromatin modifiers and negatively regulates transcription. Related references: PMID:21777816
• Cooperates with UHRF2 to ensure recruitment of FANCD2 to DNA interstrand cross-links, playing a role in DNA repair. Related references: PMID:30335751
• Also ubiquitinates non-histone proteins such as KIF11 (critical for spindle architecture) and PML, demonstrating broader regulatory functions. Related references: PMID:22945642 PMID:37728657
• Expressed in thymus, bone marrow, testis, lung, and heart; overexpressed in breast cancer. UHRF1 undergoes post-translational modifications including phosphorylation and acetylation, which may regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-260) of human UHRF1; this region contains the ubiquitin-like domain and may recognize full-length endogenous protein across human, mouse, and rat samples.
• For Western blotting, expect a major band at approximately 90 kDa; sample preparation and gel conditions may affect migration.
• For immunohistochemistry (paraffin-embedded sections), validate in tissues with known UHRF1 expression (e.g., testis, breast cancer) and use appropriate antigen retrieval.
• This antibody is suitable for ELISA; consider optimizing coating conditions and pairing with a labeled secondary antibody for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. This anti-UHRF1 antibody is part of our epigenetics portfolio, supporting DNA methylation and chromatin research applications. From bulk procurement to rare target sourcing, our team ensures seamless integration into your assay development workflow—from concept to clinic.