Anti-UHRF1 Polyclonal Antibody for WB, ELISA - Q96T88

Core Product Specifications and Parameters

Biological Background: UHRF1 Function and Localization

• UHRF1 (E3 ubiquitin-protein ligase UHRF1) is a key epigenetic regulator that bridges DNA methylation and chromatin modification.
  Related references: PMID:10646863, PMID:15009091, PMID:19056828
• It promotes recruitment of DNMT1 to hemimethylated DNA for faithful propagation of DNA methylation patterns.
  Related references: PMID:23022729, PMID:24013172, PMID:27595565
• UHRF1 specifically recognizes hemimethylated DNA and histone H3K9me3/H3R2me0 marks, activating its E3 ubiquitin ligase activity to mediate H3K18 ubiquitination (docking site for DNMT1).
  Related references: PMID:15361834, PMID:17673620, PMID:17967883, PMID:27595565
• It also mediates H3K14 and H3K23 ubiquitination at lower levels, and stimulates SUV39H1/2 methyltransferase activity promoting H3K9me3.
  Related references: PMID:24013172, PMID:27595565, PMID:39631394
• UHRF1 is enriched in pericentric heterochromatin and localizes to euchromatic regions, negatively regulating transcription.
  Related references: PMID:21777816
• It plays a role in DNA repair by cooperating with UHRF2 to recruit FANCD2 to interstrand cross-links.
  Related references: PMID:30335751
• Additionally, UHRF1 ubiquitinates non-histone proteins such as KIF11 to control spindle architecture.
  Related references: PMID:22945642, PMID:37728657
• Subcellular localization: Nucleus, Chromosome. Expressed in thymus, bone marrow, testis, lung, and heart; overexpressed in breast cancer.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-260) of human UHRF1, covering the ubiquitin-like domain; consider this when interpreting results for different isoforms or truncations.
• For Western blot (WB), validate the antibody using whole-cell lysates from human or mouse cell lines known to express UHRF1 (e.g., HeLa or NIH/3T3). A band around 90 kDa is expected.
• In ELISA, use recombinant UHRF1 protein or the immunogen fragment as a coating antigen to establish a standard curve and assess antibody binding affinity.
• As a polyclonal antibody, lot-to-lot consistency should be verified in the relevant sample system before critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material supplier, offering researchers and diagnostic manufacturers one-stop access to a broad portfolio of over 10,000 products, including validated antibody pairs, optimized polyclonal antibodies, ancillary reagents, and rare-target sourcing solutions. This anti-UHRF1 antibody, tailored for epigenetic and DNA methylation research, exemplifies our commitment to delivering high-quality tools for advanced biomedical studies. For bulk orders, custom production, or technical consultation, CamelBio provides reliable supply and expert support to accelerate your projects.