Anti-UHRF1 Polyclonal Antibody for WB and ELISA - Q96T88

Core Product Specifications and Parameters

Biological Background: UHRF1 Function and Localization

• UHRF1 (Ubiquitin-like PHD and RING finger domain-containing protein 1) is an E3 ubiquitin-protein ligase and a key epigenetic regulator that bridges DNA methylation and chromatin modification. Related references: PMID:10646863, PMID:15009091, PMID:19056828
• It plays a pivotal role in DNA methylation inheritance by recruiting DNMT1 to hemimethylated DNA at replication forks, ensuring faithful propagation of methylation patterns. Related references: PMID:23022729, PMID:24013172, PMID:27595565
• UHRF1 acts as a histone reader and writer: it recognizes H3K9me3 and unmethylated H3R2, then catalyzes monoubiquitination of H3K18 (H3K18ub) to create a docking site for DNMT1; it also ubiquitinates H3K14 and H3K23 at lower levels, coupling histone modifications with DNA methylation maintenance. Related references: PMID:15361834, PMID:17673620, PMID:17967883, PMID:27595565, PMID:24013172
• Its ubiquitin ligase activity stimulates SUV39H1/2 methyltransferases, promoting accumulation of repressive H3K9me3 marks to reinforce heterochromatin. Related references: PMID:39631394
• UHRF1 localizes to pericentric heterochromatin and euchromatic regions; it represses transcription in euchromatin, likely through its impact on DNA methylation and histone modifications. Related references: PMID:21777816
• In response to DNA damage, UHRF1 cooperates with UHRF2 to promote FANCD2 recruitment to interstrand crosslinks, facilitating DNA repair. Related references: PMID:30335751
• Beyond histones, UHRF1 ubiquitinates non-histone substrates such as KIF11 (with Lys-63 linkage) to regulate spindle architecture, and PML, extending its regulatory functions. Related references: PMID:22945642, PMID:37728657
• Tissue expression: UHRF1 is found in thymus, bone marrow, testis, lung, and heart, and is frequently overexpressed in breast cancer.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 672-793 of human UHRF1, a region that may be well-exposed for antibody binding. Consider using recombinant protein or cell lysates as positive controls.
• For western blot (WB), validate the antibody in human cell lines known to express UHRF1, such as HeLa or MCF7. The expected band size is ~90 kDa.
• In ELISA applications, this antibody can be used for direct antigen detection; optimal coating and detection conditions should be empirically determined.
• Non-specific binding may be reduced by optimizing blocking conditions and antibody dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from early-stage development through clinical application. For epigenetics-focused projects, we offer validated UHRF1 antibodies, recombinant proteins, and complementary reagents to accelerate your DNA methylation and chromatin modification studies. Our global supply network also covers bulk ancillary materials and custom sourcing for rare targets, ensuring a seamless procurement experience.