Anti-UGT3A2 Polyclonal Antibody for WB, ELISA - Q3SY77

Core Product Specifications and Parameters

Biological Background: UGT3A2 Function and Localization

• Belongs to the UDP-glycosyltransferase family and catalyzes sugar conjugation of lipophilic substrates to increase water solubility and excretion, playing a key role in xenobiotic and endobiotic metabolism.

• Utilizes UDP-glucose and UDP-xylose as primary sugar donors, and to a lesser extent UDP-galactose, but lacks activity with UDP-glucuronic acid or UDP-N-acetylglucosamine. Related references: PMID:21088224 PMID:22621930

• Accepts a broad range of acceptor substrates including 4-methylumbelliferone, 1-hydroxypyrene, bioflavones, and estrogens. Related references: PMID:21088224

• Shows negligible activity toward bile acids and androgens. Related references: PMID:21088224

• Subcellular localization: Membrane protein with a signal peptide and transmembrane helix.

• Tissue specificity: Highly expressed in thymus, testes, and kidney, with very low expression in liver and gastrointestinal tract.

• Post-translational modifications: Glycoprotein (potential N-glycosylation sites).

• Alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blot, the antibody detects a band around 60 kDa; consider using enriched membrane fractions to improve sensitivity given the membrane localization and low expression in some tissues.
• ELISA applications may require coating with recombinant UGT3A2 protein; titration of antibody concentration is advised.
• When analyzing samples from mouse or rat, perform parallel controls as cross-reactivity is indicated but not quantified.
• Optimize sample preparation with protease inhibitors and appropriate detergents for membrane protein extraction.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD manufacturers and researchers, offering a wide range of high-quality raw materials including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For targets like UGT3A2 involved in drug metabolism and toxicology, we provide tailored solutions to support assay development from concept to clinic. This UGT3A2 Rabbit pAb exemplifies our commitment to delivering reliable reagents for critical research areas.