Anti-UGT2B17 Rabbit Polyclonal Antibody for IF/ICC and ELISA - O75795

Core Product Specifications and Parameters

Biological Background: UGT2B17 Function and Localization

• UGT2B17 (UDP-glucuronosyltransferase 2B17) is a member of the UGT2B subfamily of phase II metabolizing enzymes, responsible for conjugating glucuronic acid to lipophilic substrates to enhance their water solubility.
• Catalyzes the glucuronidation of endogenous steroid hormones, including C19-steroids (androsterone, epitestosterone) and estrogens (estradiol, epiestradiol), facilitating their excretion. Related references: PMID:16595710 PMID:18719240 PMID:19022937
• Localized in the endoplasmic reticulum membrane, consistent with its role in cellular detoxification and lipid/steroid metabolism.
• Expressed in a broad range of tissues, including liver, kidney, testis, uterus, placenta, mammary gland, adrenal gland, skin, and prostate, as indicated by UniProt tissue specificity annotations.
• Post-translationally modified as a glycoprotein with a signal peptide and a single transmembrane helix, anchoring it to the ER membrane.
• According to UniProt keywords, UGT2B17 is involved in lipid metabolism, steroid metabolism, and has been identified in proteomics studies, underscoring its functional relevance in human biology.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 50–200 within the N-terminal region, which includes part of the signal peptide and early catalytic domain; the resulting polyclonal antibody may recognize multiple epitopes, offering robust detection.
• For immunofluorescence/ICC, consider permeabilization protocols to access the ER membrane-localized target and validate staining with an appropriate ER marker.
• In ELISA, test a dilution series against recombinant UGT2B17 protein or tissue lysates; liver microsomes or steroidogenic tissue extracts may serve as positive controls due to high expression.
• Given the broad tissue distribution, confirm specificity in your sample system using a negative control (e.g., UGT2B17-depleted lysate or knockout model) to distinguish specific signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the sourcing of high-quality IVD raw materials, offering a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This UGT2B17 rabbit polyclonal antibody is well-suited for researchers investigating steroid metabolism, pharmacogenetics, and endocrine function, and we can assist with customized procurement for rare target raw materials to support your assay development from concept to clinic.