Anti-UGT2B10 Rabbit Polyclonal Antibody for WB and ELISA - P36537

Core Product Specifications and Parameters

Biological Background: UGT2B10 Function and Localization

• UGT2B10 catalyzes phase II biotransformation reactions, conjugating glucuronic acid to lipophilic substrates to enhance water solubility and excretion (PubMed:17576790, PubMed:18238858, PubMed:28803208).
  Related references: PMID:17576790 PMID:18238858 PMID:28803208

• Specializes in N-glucuronidation of tertiary amine-containing drugs and xenobiotics, including tobacco alkaloids and tricyclic antidepressants (PubMed:20133892, PubMed:28803208).
  Related references: PMID:20133892 PMID:28803208

• Acts as the primary enzyme for detoxifying tobacco-specific nitrosamines, critical in reducing carcinogenic burden (PubMed:18238858, PubMed:18300939).
  Related references: PMID:18238858 PMID:18300939

• Exhibits strong preference for tertiary aliphatic and cyclic amines over secondary or primary amines; does not glucuronidate steroids, hyodeoxycholic acid, or phenolic xenobiotics (PubMed:20133892, PubMed:28803208, PubMed:8333863).
  Related references: PMID:20133892 PMID:28803208 PMID:8333863

• Localizes to the endoplasmic reticulum and microsomal membranes, consistent with phase II metabolic function.

• Highly expressed in the liver, with almost no expression in other tissues.

• Encodes alternative splice variants, glycosylation sites, and a transmembrane helix, classifying it as a reference proteome transferase.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using reducing/denaturing conditions and probing liver tissue lysates from human, mouse, or rat to verify the expected ~61 kDa band; optimize antibody dilution empirically.
• In ELISA, the polyclonal antibody may serve as a capture or detection reagent; validate performance with recombinant UGT2B10 protein or native samples.
• Because the immunogen spans amino acids 20–280, the antibody likely recognizes multiple UGT2B10 isoforms and orthologs in rodents; confirm cross-reactivity in your specific experimental system.
• As with any polyclonal reagent, lot-to-lot consistency should be monitored; consider including a reference standard in each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to UGT2B10 antibodies and related IVD raw materials for drug metabolism and toxicology research. We supply validated polyclonal and monoclonal antibodies, optimized antibody pairs, bulk ancillary reagents, and can source rare targets to streamline your assay development. Trust CamelBio as your procurement partner from concept to clinic.