Anti-UGT1A6 Polyclonal Antibody for WB, IF-P, ELISA - P19224

Core Product Specifications and Parameters

Biological Background: UGT1A6 Function and Localization

• UGT1A6 is a UDP-glucuronosyltransferase that catalyzes phase II biotransformation, conjugating lipophilic substrates with glucuronic acid for elimination and detoxification of drugs, xenobiotics, and endogenous compounds. Related references: PMID:15231852 PMID:21422672
• It plays a role in the glucuronidation of arachidonic acid and its eicosanoid derivatives, such as 15-HETE and 20-HETE. Related references: PMID:15231852
• The enzyme preferentially conjugates small planar phenolic molecules (e.g., 4-nitrophenol, 1-naphthol) but not bulky phenols, androgens, or estrogens. 2-hydroxybiphenyl is an excellent substrate.
• UGT1A6 is involved in glucuronidation of the phytochemical ferulic acid at the phenolic or carboxylic acid group. Related references: PMID:21422672
• Alternative splicing produces isoform 3, which lacks transferase activity but acts as a negative regulator of isoform 1.
• Subcellularly, UGT1A6 localizes to the microsome and endoplasmic reticulum membrane, consistent with its role in detoxification pathways.
• Tissue expression includes skin; isoforms 1 and 3 are found in kidney and liver; isoform 1 is also detected in colon, esophagus, and small intestine.
• Post-translational modifications include glycosylation, and it contains a signal sequence and transmembrane helix, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• For Western blotting, consider using a suitable loading control and validate band specificity with knockdown or knockout controls.
• Given the immunogen design covering the core glycosyltransferase domain, the antibody may recognize multiple UGT1A isoforms sharing high sequence homology; verify specificity in your experimental system.
• For immunofluorescence, permeabilization may be required to access the ER membrane, and include appropriate negative controls to confirm signal specificity.
• In ELISA applications, optimize coating conditions and antibody dilutions using relevant recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive IVD raw material solutions, from antibody development to bulk reagent supply. For UGT1A6 and other phase II metabolism targets, we offer optimized polyclonal antibodies, validated antibody pairs, and ancillary reagents to accelerate assay development. Our one-stop sourcing model ensures seamless access to rare target raw materials and technical consulting for your pipeline.