Anti-UGT1A6 Polyclonal Antibody for WB, ELISA - P19224

Core Product Specifications and Parameters

Biological Background: UGT1A6 Function and Localization

• UGT1A6 is a UDP-glucuronosyltransferase (UGT) that catalyzes the conjugation of glucuronic acid to lipophilic substrates, facilitating their elimination and detoxification, including drugs, xenobiotics, and endogenous compounds like arachidonic acid and its eicosanoid metabolites (15-HETE, 20-HETE). Related references: PMID:15231852 PMID:21422672
• The enzyme preferentially glucuronidates small planar phenolic molecules such as 4-nitrophenol, 1-naphthol, and 4-methylumbelliferone, but not bulky phenols or steroids; 2-hydroxybiphenyl is an excellent substrate.
• UGT1A6 belongs to the UGT1A gene family (alternative gene symbols: GNT1, UGT1), which encodes multiple isoforms through alternative splicing and promoter usage; the protein is also known as UDPGT 1-6, UGT-1F, and phenol-metabolizing UDP-glucuronosyltransferase.
• UGT1A6 localizes to the endoplasmic reticulum membrane and microsomal fraction, consistent with its role in phase II metabolism.
• The protein is subject to alternative splicing, yielding at least three isoforms; isoform 3 lacks catalytic activity and negatively regulates isoform 1 function.
• Post-translational modifications include glycosylation, and the protein features a signal peptide and a transmembrane helix.
• Tissue expression is detected in skin, kidney, liver, colon, esophagus, and small intestine, with isoform-specific distribution patterns.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 65-270, a region likely to be accessible on the native protein, but consider that reactivity may be influenced by post-translational modifications or isoforms.
• For Western blot, a band at approximately 61 kDa is expected; validate specificity using appropriate positive and negative controls (e.g., UGT1A6-overexpressing cells or knockdown samples).
• In ELISA, coating antigen and detection conditions should be optimized; cross-reactivity with mouse UGT1A6 may assist in translational studies but requires confirmation in the relevant biological context.
• Given the polyclonal nature, lot-to-lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated sourcing partner for IVD manufacturers and research laboratories, offering comprehensive access to raw materials for drug metabolism and pharmacokinetic assay development. We supply optimized antibodies like the UGT1A6 polyclonal antibody, along with validated antibody pairs, recombinant proteins, and ancillary reagents in bulk quantities. Our global procurement network also supports the sourcing of rare and niche targets, streamlining your development from discovery to diagnostic application.