Anti-UGT1A6 Polyclonal Antibody for WB, ELISA - P19224

Core Product Specifications and Parameters

Biological Background: UGT1A6 Function and Localization

• UGT1A6 is a member of the UGT1 family and functions as a phenol-metabolizing UDP-glucuronosyltransferase (UDPGT) critical for the detoxification of drugs, xenobiotics, and endogenous compounds by conjugating them with glucuronic acid. Related references: PMID:15231852, PMID:21422672
• Exhibits broad substrate specificity towards small planar phenols, including 4-nitrophenol, 1-naphthol, and 4-methylumbelliferone, but does not conjugate bulky phenols or steroid hormones.
• Involved in the glucuronidation of the dietary phytochemical ferulic acid, utilizing both phenolic and carboxylic acid groups. Related references: PMID:21422672
• Plays a role in the glucuronidation of arachidonic acid and its derivatives 15-HETE and 20-HETE, linking it to eicosanoid metabolism.
• Isoform 3, generated by alternative splicing, lacks transferase activity and acts as a negative regulator of isoform 1.
• Primarily localized in the membrane of the endoplasmic reticulum and microsomes.
• Tissue expression includes liver, kidney, skin, colon, esophagus, and small intestine, with isoform-dependent distribution.
• Contains N-glycosylation sites and a transmembrane domain, consistent with its membrane-bound transferase function.

Experimental Guidance and Technical Tips

• For Western blot, expect a specific band around 61 kDa (predicted molecular weight). Use reducing conditions and validate with appropriate positive controls (e.g., human liver microsomes or HepG2 cell lysates).
• Since polyclonal antibodies may recognize multiple isoforms, consider confirming isoform specificity by consulting sequence alignment and testing in isoform-expressing systems.
• For ELISA, the immunogen corresponds to amino acids 65-270; ensure the coating antigen includes this region. Perform checkerboard titrations to optimize antibody and antigen concentrations.
• Cross-reactivity has been detected with human and mouse; if extending to other species, validate sequence homology of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw material sourcing, offering a comprehensive portfolio that includes validated antibody pairs, recombinant proteins, and ancillary reagents. Our UGT1A6 polyclonal antibody supports metabolism and detoxification research, and we can also assist with bulk custom orders and rare target raw-material sourcing to accelerate your assay development from concept to clinic.