Anti-UGT1A1 Rabbit Monoclonal Antibody for WB, ELISA - P22309

Core Product Specifications and Parameters

Biological Background: UGT1A1 Function and Localization

• Encodes UGT1A1, a phase II conjugating enzyme that adds glucuronic acid to lipophilic substrates, facilitating their excretion. Related references: PMID:12181437, PMID:15472229, PMID:18004206, PMID:18004212
• Crucial for bilirubin detoxification; genetic variants are associated with hyperbilirubinemia syndromes. Related references: PMID:17187418, PMID:18004206, PMID:19830808
• Metabolizes endogenous steroids (estrogens) and lipid mediators (eicosanoids), impacting hormonal and inflammatory processes. Related references: PMID:15472229, PMID:18719240, PMID:23288867, PMID:15231852, PMID:38211441
• Glucuronidates therapeutic drugs such as losartan and SN-38, modulating their efficacy and safety. Related references: PMID:18674515, PMID:12181437, PMID:18004212, PMID:20610558
• Conjugates dietary phytoestrogens (genistein) and phenolic acids (ferulic acid), influencing their bioactivity. Related references: PMID:21422672, PMID:18052087, PMID:19545173
• Resides in the endoplasmic reticulum membrane; an alternative splice form acts as a non‑catalytic regulator.
• Predominantly expressed in liver, colon, and small intestine, with some isoforms in kidney.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal peptide (aa 101–200), suggesting the antibody may detect both wild‑type and some variants. Consider epitope mapping if precise isoform discrimination is required.
• For Western blot, a band near 60 kDa is expected; extended electrophoresis or deglycosylation may resolve mobility shifts due to glycosylation.
• In ELISA, using recombinant UGT1A1 protein or lysates from human liver/hepatocyte lines is recommended; cross‑reactivity with rat should be confirmed in the relevant tissue.
• Validate the antibody’s performance in your specific application and sample type, as endogenous expression levels can vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers IVD developers with a comprehensive portfolio of high-quality raw materials, including validated antibody pairs, optimized recombinant antibodies, and bulk ancillary reagents. This UGT1A1-specific antibody supports critical research and assay development in drug metabolism, hepatotoxicity, and bilirubin-related disorders. Explore our one-stop sourcing bridge to accelerate your diagnostic projects from concept to clinic.