Anti-UGT1A1 Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P22309

Core Product Specifications and Parameters

Biological Background: UGT1A1 Function and Localization

• UGT1A1 (UDP-glucuronosyltransferase 1A1), also known as HUG-BR1 or UGT1*1, is a key phase II biotransformation enzyme that conjugates glucuronic acid to lipophilic substrates, increasing their water solubility for excretion into urine or bile (PubMed:12181437, PubMed:18004206). It is essential for the elimination and detoxification of drugs, xenobiotics, and endogenous compounds (PubMed:12181437, PubMed:18004206, PubMed:18004212). Related references: PMID:12181437 PMID:18004206 PMID:18004212

• Plays a critical role in bilirubin metabolism by catalyzing its glucuronidation, a vital step in the heme catabolic pathway; genetic deficiency is associated with hyperbilirubinemia syndromes (PubMed:17187418, PubMed:18004206, PubMed:19830808). Related references: PMID:17187418 PMID:18004206 PMID:19830808

• Mediates the glucuronidation of endogenous estrogens (estradiol, estrone, estriol), thereby regulating hormone levels and activity (PubMed:15472229, PubMed:18719240, PubMed:23288867). Related references: PMID:15472229 PMID:18719240 PMID:23288867

• Participates in the metabolism of the anticancer drug irinotecan by converting its active metabolite SN-38 into an inactive glucuronide, influencing drug efficacy and toxicity (PubMed:12181437, PubMed:18004212, PubMed:20610558). Related references: PMID:12181437 PMID:18004212 PMID:20610558

• Catalyzes the glucuronidation of the angiotensin receptor blocker losartan, affecting its pharmacokinetics (PubMed:18674515). Related references: PMID:18674515

• Involved in the conjugation of arachidonic acid and eicosanoids, including 15-HETE, 20-HETE, and F2-isoprostane, linking UGT1A1 to inflammatory and oxidative stress pathways (PubMed:15231852, PubMed:38211441). Related references: PMID:15231852 PMID:38211441

• Modulates the bioavailability of dietary phytochemicals and phytoestrogens, such as ferulic acid and isoflavones (genistein, daidzein), through glucuronidation (PubMed:21422672, PubMed:18052087, PubMed:19545173). Related references: PMID:21422672 PMID:18052087 PMID:19545173

• The UGT1A1 gene undergoes alternative splicing, generating isoforms; some may lack enzymatic activity but act as negative regulators of the active enzyme.

• Localized predominantly in the endoplasmic reticulum membrane and perinuclear cytoplasmic region, consistent with its role in cellular detoxification.

• Highly expressed in the liver, colon, and small intestine, with lower or absent expression in kidney, esophagus, and skin, depending on the isoform.

• Post-translational modifications include glycosylation, and the protein features a signal peptide and a single transmembrane domain, classifying it as a type I membrane protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-200) of human UGT1A1; this region may recognize multiple isoforms or splice variants. Verify specificity in the target tissue or cell line of interest.
• For Western blot, the predicted molecular weight is approximately 60 kDa. Use suitable positive controls such as human liver microsomes or UGT1A1-overexpressing cell lysates. Optimize antibody dilution for your specific samples.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC, IF-P), consider antigen retrieval methods and use human liver or colon sections as positive controls. Validate co-localization with an ER marker if studying subcellular distribution.
• This antibody is reported to cross-react with mouse and rat UGT1A1. For animal studies, confirm cross-reactivity in the relevant species tissues, as sequence homology may affect detection sensitivity.
• ELISA protocol development may require antibody pair screening; test for compatibility with your antigen coating and detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes streamlined access to this UGT1A1 polyclonal antibody, providing robust performance for WB, IHC, IF, and ELISA applications. As a specialized IVD raw material partner, we support your drug metabolism, toxicology, and bilirubin disorder research with end-to-end sourcing—from validated antibody pairs and optimized bulk reagents to rare-target procurement. Partner with CamelBio to accelerate your assay development with reliable, high-quality immunological tools, backed by technical and supply chain expertise.