Anti-UGT1A1 Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P22309

Core Product Specifications and Parameters

Biological Background: UGT1A1 Function and Localization

• UGT1A1 is a UDP-glucuronosyltransferase that catalyzes phase II conjugation of glucuronic acid to lipophilic substrates, facilitating their excretion (PubMed:12181437, PubMed:15472229, PubMed:18004206). Related references: PMID:12181437, PMID:15472229, PMID:18004206
• Essential for bilirubin detoxification, it converts unconjugated bilirubin to water-soluble glucuronides for biliary elimination (PubMed:17187418, PubMed:18004206, PubMed:19830808). Related references: PMID:17187418, PMID:18004206, PMID:19830808
• Glucuronidates endogenous estrogens (estradiol, estrone, estriol), regulating their bioavailability (PubMed:15472229, PubMed:18719240, PubMed:23288867). Related references: PMID:15472229, PMID:18719240, PMID:23288867
• Metabolizes the irinotecan active metabolite SN-38, influencing cancer drug efficacy and toxicity (PubMed:12181437, PubMed:18004212, PubMed:20610558). Related references: PMID:12181437, PMID:18004212, PMID:20610558
• Localizes to the endoplasmic reticulum membrane and perinuclear cytoplasm.
• Highly expressed in liver, colon, and small intestine; isoform-specific expression in kidney.
• Alternative splicing generates isoforms lacking enzymatic activity that may negatively regulate UGT1A1 function. Mutations are linked to inheritable hyperbilirubinemia syndromes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-200), which shares high homology among UGT1A family members; cross-reactivity with related isoforms (e.g., UGT1A3, UGT1A4) should be assessed.
• For Western blot, the predicted molecular weight is ~60 kDa; endogenous UGT1A1 levels may be low in many cell lines, so enrichment or overexpression may be required.
• In IHC-P, liver and intestinal tissues are recommended as positive controls; optimize antigen retrieval conditions.
• For IF/ICC, note the ER membrane localization; co-staining with ER markers can validate specificity.
• ELISA applications may benefit from pairing this polyclonal with a monoclonal capture antibody in a sandwich format.
• As a polyclonal antibody, lot-to-lot consistency should be verified in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material sourcing for diagnostic developers and research laboratories. For UGT1A1-related studies in drug metabolism and bilirubin conjugation, we offer validated antibody pairs, bulk ancillary reagents, and access to rare-target raw materials. Our one-stop platform supports your journey from concept to clinic.