Anti-UGGT1 Rabbit Polyclonal Antibody for WB, ELISA - Q9NYU2

Core Product Specifications and Parameters

Biological Background: UGGT1 Function and Localization

• UGGT1 (UDP-glucose:glycoprotein glucosyltransferase 1), also known as UGCGL1, HUGT1, or UGT1, is a ~177 kDa glycoprotein and member of the glycosyltransferase family, encoded by the UGGT1 gene in humans.
• It functions as a key quality control enzyme in the endoplasmic reticulum (ER), recognizing glycoproteins with minor folding defects and reglucosylating single N-glycans near the misfolded region to enable calreticulin-mediated recycling for refolding or degradation.
• UGGT1 is primarily localized to the ER lumen and the ER-Golgi intermediate compartment, ensuring continuous surveillance of protein folding along the secretory pathway.
• The protein is subject to alternative splicing and phosphorylation; mutations and variants in UGGT1 are associated with congenital disorders of glycosylation.
• Tissue expression studies reveal higher UGGT1 levels in pancreas, skeletal muscle, kidney, and brain, with lower levels in lung and heart, reflecting tissue-specific ER protein quality control demands.

Experimental Guidance and Technical Tips

• The immunogen targets a C-terminal region (aa 1456-1555) of human UGGT1; for maximum specificity, consider validating with knockout or knockdown controls in your sample system.
• For Western blotting, optimize antibody dilution based on your sample type and detection system; expect a band around 177 kDa. Ensure adequate denaturation (boiling with reducing agent) and consider using freshly prepared lysates to minimize degradation.
• Cross-reactivity is demonstrated for human, mouse, and rat; when applying to other species, perform sequence alignment to assess homology before use.
• The antibody is also suitable for ELISA; performance may vary with coating conditions and detection methods, so appropriate optimization is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material procurement, offering a streamlined path from concept to clinic. For researchers focused on ER quality control, glycoprotein folding, and related diseases, we provide validated antibodies like this anti-UGGT1 pAb, along with ancillary reagents, recombinant proteins, and sourcing support for rare targets. Let us bridge your development needs with reliable supply and technical expertise.