Anti-UGDH Polyclonal Antibody for IHC-P and ELISA - O60701

Core Product Specifications and Parameters

Biological Background: UGDH Function and Localization

• UGDH (UDP-glucose 6-dehydrogenase) catalyzes the conversion of UDP-glucose to UDP-glucuronate, a critical precursor for glycosaminoglycans including chondroitin sulfate and heparan sulfate.
  Related references:
  PMID:21502315 PMID:21961565 PMID:22123821

• Essential for embryonic development through its role in glycosaminoglycan biosynthesis; also required for proper brain and neuronal development.
  Related references:
  PMID:32001716

• Functions as an allosteric enzyme regulated by NAD and is subject to acetylation and phosphorylation.

• Alternative splicing generates multiple isoforms; mutations in UGDH are linked to developmental and epileptic encephalopathies (DEE84/EIEE84).

• Ubiquitously expressed with high levels detected in heart, placenta, liver, pancreas, spleen, thymus, prostate, ovary, small intestine, and colon.

• Localizes to the cytoplasm where it participates in carbohydrate metabolism.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 165–494 of human UGDH, a region well conserved in mouse and rat, ensuring broad species cross-reactivity.
• For IHC-P, consider heat-induced antigen retrieval and validate with positive control tissues such as liver or placenta.
• In ELISA, optimize coating buffer and incubation times to achieve optimal signal-to-noise ratio.
• The polyclonal nature of the antibody may allow adaptation to other techniques like Western blotting (predicted band ~55 kDa); however, individual validation is essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for rare-target sourcing. For UGDH-related research in glycosaminoglycan metabolism, neurodevelopment, and genetic disorders, we provide integrated sourcing solutions that move your project from concept to clinical application efficiently.