Anti-UFL1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O94874

Core Product Specifications and Parameters

Biological Background: UFL1 Function and Localization

• UFL1 functions as an E3 protein ligase that mediates ufmylation—the covalent attachment of the ubiquitin-like modifier UFM1 to lysine residues on target proteins—playing key roles in ribosome recycling, DNA damage response, interferon signaling, and reticulophagy (ER-phagy).
  Related references: PMID:20018847, PMID:20164180, PMID:20228063

• As part of the UREL complex, UFL1 catalyzes mono-ufmylation of the 60S ribosomal subunit protein RPL26/uL24, which promotes ribosome recycling and release from the endoplasmic reticulum membrane both after normal translation termination and upon ribosome stalling during cotranslational translocation.
  Related references: PMID:38383785, PMID:38383789, PMID:37036982, PMID:37595036

• UFL1 promotes reticulophagy in response to endoplasmic reticulum stress by ufmylating substrates such as CYB5R3 and RPN1, targeting them for lysosomal degradation; this process is essential for hematopoiesis, blood vessel morphogenesis, and inflammatory responses.
  Related references: PMID:23152784, PMID:32160526, PMID:36543799, PMID:32050156

• In the DNA damage response, UFL1 is recruited to double-strand break sites and mediates monoufmylation of histone H4 as well as ufmylation of MRE11, connecting the ufmylation pathway to DNA repair.
  Related references: PMID:30783677, PMID:30886146

• UFL1 acts as a negative regulator of T-cell activation by mediating ufmylation and stabilization of the immune checkpoint receptor PD-1, and also ufmylates TP53/p53 to promote its stability.
  Related references: PMID:38377992, PMID:32807901

• Post-translational modifications of UFL1 include acetylation, methylation, and phosphorylation. UFL1 is ubiquitously expressed, with highest levels in the liver, and shows reduced expression in several invasive hepatocellular carcinomas such as Hep-G2 and HLE.
  Related references: PMID:20018847

• UFL1 localizes to the endoplasmic reticulum membrane, cytoplasm, cytosol, nucleus, and chromosomes, consistent with its diverse roles in ribosomal function, ER quality control, and DNA repair.

Experimental Guidance and Technical Tips

• The immunogen comprising amino acids 601–794 of human UFL1 is located in the C-terminal region, which may enhance target specificity; consider verifying reactivity in your sample matrix by including appropriate controls.
• For Western blotting (WB), a major band at approximately 90 kDa is anticipated. Optimize blocking and antibody dilution using lysates from human, mouse, or rat cells known to express UFL1.
• In immunofluorescence/immunocytochemistry (IF/ICC), UFL1 can display multi-compartment localization (ER, cytoplasm, nucleus). Fixation and permeabilization conditions should be empirically optimized to preserve epitope recognition.
• For ELISA applications, the antibody can be employed as a capture or detection reagent for UFL1 or ufmylated substrates; validate assay specificity and sensitivity with relevant standards.
• As a rabbit polyclonal antibody, it recognizes multiple epitopes; consider its potential utility in immunoprecipitation or other techniques after individual validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined one-stop source for IVD raw materials, technical services, and consulting. This anti-UFL1 rabbit polyclonal antibody is an example of our high-quality immunological reagents designed for research into ufmylation, ER stress, and genome stability. With capabilities spanning validated antibody pairs, bulk production of monoclonal and polyclonal antibodies, and sourcing of rare targets, CamelBio supports your pathway from early-stage discovery to clinical assay development.