Anti-UFD1L Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q92890

Core Product Specifications and Parameters

Biological Background: UFD1L Function and Localization

• UFD1L (Ubiquitin fusion degradation protein 1-like), also known as Ub fusion protein 1, is an essential component of the ubiquitin-dependent proteolytic pathway that degrades ubiquitin fusion proteins.
• It forms a ternary complex with VCP/p97 and NPLOC4, which recognizes ubiquitinated substrates and facilitates the retrotranslocation and export of misfolded proteins from the endoplasmic reticulum (ER) to the cytoplasm for proteasomal degradation.
• The UFD1-VCP-NPLOC4 complex also plays a critical role in mitotic spindle disassembly at the end of mitosis and is required for closed nuclear envelope formation.
• UFD1L negatively regulates type I interferon production by forming a complex with VCP and NPLOC4 that binds to RIG-I, recruiting the E3 ubiquitin ligase RNF125 to promote ubiquitination and subsequent degradation of RIG-I. Related references: PMID:26471729
• Subcellularly, UFD1L is found in the nucleus, cytoplasm, and predominantly in the cytosol.
• Tissue expression includes adult heart, skeletal muscle, and pancreas, as well as fetal liver and kidney.
• Post-translational modifications include acetylation and phosphorylation; the UFD1 gene undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen region (aa 1-307) covers a large N-terminal portion of UFD1L, which may enable detection of multiple isoforms; however, validate specificity in the relevant sample system.
• For Western blot analysis, expect a band near the predicted molecular weight of 35 kDa (34.5 kDa theoretical). Use appropriate positive controls (e.g., HeLa, HEK293).
• In immunofluorescence/ICC, optimize fixation and permeabilization conditions, as UFD1L localizes to both nuclear and cytoplasmic compartments.
• For ELISA applications, use the recombinant immunogen or purified protein as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing platform for IVD raw materials, supporting diagnostic manufacturers and research labs from development to production. Whether you require validated antibody pairs, optimized polyclonal or monoclonal antibodies, or bulk ancillary reagents, our streamlined supply chain can meet your needs. For UFD1L-related research in ER-associated degradation and ubiquitin pathways, we provide high-quality antibodies and can source rare-target reagents to accelerate your assay development.