Anti-UFC1 Rabbit Monoclonal Antibody for WB, ELISA - Q9Y3C8

Core Product Specifications and Parameters

Biological Background: UFC1 Function and Localization

• UFC1 (Ubiquitin-fold modifier-conjugating enzyme 1) is an E2-like enzyme that functions in the ufmylation pathway.
• It catalyzes the second step in ufmylation by accepting UFM1 from the E1 enzyme UBA5 and forming a thioester-linked intermediate. Related references: PMID:15071506, PMID:29868776, PMID:30626644, PMID:34588452
• Ufmylation mediated by UFC1 plays roles in ribosome recycling, response to DNA damage, interferon signaling, and reticulophagy. Related references: PMID:27351204, PMID:32160526, PMID:35394863
• The protein has a calculated molecular mass of approximately 19.5 kDa and is involved in the Ubl conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–130 of human UFC1; consider its potential to recognize full-length and N-terminal variants in lysates.
• For Western blot, a band near 19 kDa is expected; validate in human, mouse, and rat samples as cross-reactivity is reported.
• For ELISA, optimize direct or sandwich formats empirically; no pre-validated dilution is supplied.
• Determine optimal working dilutions for each application using a titration series under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with one-stop access to validated IVD raw materials, including this anti-UFC1 monoclonal antibody for ufmylation and stress response studies. We supply optimized antibody pairs, bulk ancillary reagents, and support rare-target sourcing to streamline your assay development from concept to clinic.