Anti-UE2B Monoclonal Antibody for WB, IHC-P, ELISA - P63146

Core Product Specifications and Parameters

Biological Background: UBE2B Function and Localization

• UBE2B (Ubiquitin-conjugating enzyme E2 B), also known as RAD6B or HR6B, is a member of the ubiquitin-conjugating enzyme family that functions as an E2 enzyme in the ubiquitin-proteasome system.
• It plays a critical role in histone H2B monoubiquitination at Lys-120 (H2BK120ub1), which is an epigenetic mark for transcriptional activation, elongation by RNA polymerase II, and telomeric silencing. Related references: PMID:16337599
• In the DNA damage response, UBE2B partners with the E3 ligase RAD18 to monoubiquitinate PCNA at Lys-164, promoting post-replication DNA repair. Related references: PMID:17108083 PMID:17130289
• In vitro, UBE2B catalyzes the formation of Lys-11-, Lys-48-, and Lys-63-linked polyubiquitin chains, indicating its versatility in ubiquitin chain topology. Related references: PMID:20061386
• UBE2B is also involved in N-end rule-dependent protein degradation through association with the E3 enzyme UBR4. Related references: PMID:38182926
• The protein localizes to both the cell membrane and the nucleus, consistent with its roles in signaling and chromatin modification.
• Additionally, UBE2B may contribute to neuronal development, including neurite outgrowth.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 53–152 of human UBE2B; the antibody is expected to recognize an internal epitope conserved across human, mouse, and rat.
• For Western blot, a band at approximately 17 kDa is predicted; validate the antibody with appropriate positive and negative controls in your specific sample system.
• This antibody is listed for use in ELISA; consider optimizing coating and detection conditions for your specific assay format.
• In IHC-P, standard antigen retrieval methods may be required; verify staining patterns against known subcellular localizations (nucleus and membrane).
• Since UBE2B is involved in ubiquitination pathways, treatments that modulate ubiquitin levels (e.g., proteasome inhibitors) may alter detection signals; plan controls accordingly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw materials, providing a seamless sourcing experience from discovery to commercial manufacturing. With expertise in ubiquitin pathway targets like UBE2B, we supply validated monoclonal antibodies, antibody pairs, and ancillary reagents to support your assay development and research needs. Whether you require bulk quantities or rare-target sourcing, our team offers tailored solutions to accelerate your project.