Anti-UCP3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P55916

Core Product Specifications and Parameters

Biological Background: UCP3 Function and Localization

• UCP3 (also known as SLC25A9) is a member of the mitochondrial solute carrier family 25, characterized by multiple transmembrane domains.
• Its precise molecular function remains under investigation; current studies indicate a role in mitochondrial metabolism through an as yet unclear mechanism. Related references: PMID:21775425, PMID:36114012
• Historically, UCP3 was proposed to act as a proton leak channel, dissipating the proton gradient across the inner mitochondrial membrane to uncouple ATP synthesis and generate heat. Related references: PMID:11171965, PMID:12670931, PMID:12734183
• This uncoupling function is controversial; more recent evidence suggests UCP3 may instead function as a metabolite transporter, with proton leak being a secondary effect. Related references: PMID:11707458
• UCP3 localizes to the mitochondrial inner membrane, consistent with its role in mitochondrial transport.
• Expression is highly tissue-specific, found predominantly in skeletal muscle and heart, with lower levels in white and brown adipose tissue; within muscle, glycolytic fibers show higher expression than oxidative fibers. Related references: PMID:9305858
• Clinically, UCP3 has been linked to obesity and diabetes mellitus, and alternative splicing generates multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-110), which may be suitable for detecting both full-length and N-terminal fragments; consider epitope accessibility in fully folded native protein.
• Predicted molecular weight is 34 kDa, but due to the hydrophobic nature, actual migration in SDS-PAGE may differ; validate in the relevant sample system.
• For Western blot (WB), consider using mitochondrial-enriched fractions from skeletal muscle or heart tissue as positive controls, as these tissues express UCP3 at high levels.
• In immunofluorescence (IF/ICC), a mitochondrial staining pattern is expected; include mitochondrial markers for co-localization.
• Cross-reactivity with mouse and rat enables multi-species comparative studies; however, verify reactivity in your experimental model.
• For ELISA, the polyclonal nature provides broad epitope recognition, but assay optimization for sensitivity and specificity is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For metabolic disease research, our one-stop sourcing services cover targets like UCP3 involved in mitochondrial function, obesity, and diabetes. Whether you need custom antibody development or rare-target raw-material procurement, we provide flexible, traceable supply solutions from concept to clinic.