Anti-UCP2 Rabbit Polyclonal Antibody for WB, ELISA - P55851

Core Product Specifications and Parameters

Biological Background: UCP2 Function and Localization

• UCP2, encoded by the UCP2 gene (GeneID: 7351), is a mitochondrial carrier protein that functions as an antiporter exporting dicarboxylate Krebs cycle intermediates (e.g., oxaloacetate) in exchange for phosphate and a proton, thereby downregulating acetyl-CoA oxidation and reducing reactive oxygen species production. Related references: PMID:24395786
• It may mediate inducible proton entry into the mitochondrial matrix, affecting ATP turnover and protecting against oxidative stress, a process likely associated with fatty acid flipping across the inner membrane. Related references: PMID:11171965, PMID:11278935, PMID:22524567
• UCP2 can unidirectionally transport anions including L-malate, L-aspartate, phosphate, and chloride ions. Related references: PMID:22524567, PMID:24395786, PMID:26182433
• It is required for glucose-induced DRP1-dependent mitochondrial fission and neuronal activation in the ventromedial hypothalamus, contributing to systemic glucose homeostasis.
• During the resolution of inflammation, UCP2 attenuates ROS production in response to IL33, preserving Krebs cycle integrity for itaconate production and promoting differentiation of alternatively activated macrophages.
• UCP2 localizes to the mitochondrial inner membrane and is widely expressed in adult human tissues, with highest levels in white adipose tissue and skeletal muscle.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 112–211 of human UCP2, a region that may be conserved across species, potentially contributing to the observed cross-reactivity with mouse and rat.
• For Western blot, a band around 33 kDa is expected; validate using appropriate controls and reference lysates from target tissues.
• ELISA applications may require optimization of coating conditions and antibody dilution; consider testing with recombinant UCP2 protein or peptide.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined sourcing platform for high-quality IVD raw materials. This UCP2 antibody supports investigations into mitochondrial metabolism, oxidative stress, and inflammation, complementing our portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement solutions.