Anti-UCP1+UCP3 Rabbit Monoclonal Antibody for IF-P, IHC-P, ELISA - P12242 / P56501

Core Product Specifications and Parameters

Biological Background: UCP1 and UCP3 Function and Localization

• UCP1, also known as thermogenin or solute carrier family 25 member 7 (SLC25A7), is a mitochondrial inner membrane uncoupling protein.
• It functions as a long-chain fatty acid/proton symporter, dissipating the mitochondrial proton gradient to convert oxidation energy into heat rather than ATP. Related references: PMID:23063128
• UCP1 mediates thermogenic respiration in brown and beige adipocytes, playing a critical role in non-shivering adaptive thermogenesis and whole-body energy balance. Related references: PMID:19187776, PMID:23063128, PMID:27027295
• It negatively regulates mitochondrial reactive oxygen species (ROS) production. Related references: PMID:20416274, PMID:20466728
• Expressed predominantly in brown adipose tissue.
• Localizes to the mitochondrial inner membrane.
• Belongs to the mitochondrial carrier protein family; contains transmembrane domains and selenocysteine residues.

Experimental Guidance and Technical Tips

• The immunogen was generated using a recombinant fusion protein covering amino acids 1-307 of mouse UCP1 and UCP3. Ensure that detection in your experimental system accounts for both proteins.
• For immunohistochemistry (IHC-P), antigen retrieval may be necessary to expose the mitochondrial membrane epitopes; validate with appropriate controls.
• In immunofluorescence (IF-P), permeabilization is required for mitochondrial staining; co-staining with organelle markers is recommended.
• ELISA applications can be used for quantification; establish a standard curve with recombinant UCP1/UCP3 protein.
• This antibody reacts with mouse and rat; cross-reactivity with other species should be validated independently.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio supports diagnostic developers and research labs from concept to clinic. This anti-UCP1+UCP3 rabbit monoclonal antibody serves metabolic research and brown adipose tissue studies. Beyond this product, we supply validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and rare target sourcing to meet your assay development needs.