Anti-UCP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF-P, ELISA - P25874

Core Product Specifications and Parameters

Biological Background: UCP1 Function and Localization

• UCP1, also known as Thermogenin or Solute carrier family 25 member 7 (SLC25A7), is a mitochondrial uncoupling protein specifically expressed in brown adipose tissue.
• It mediates thermogenic respiration, dissipating the mitochondrial proton gradient to convert energy from substrate oxidation into heat instead of ATP, playing a critical role in non-shivering adaptive thermogenesis.
• UCP1 functions as a long-chain fatty acid (LCFA)/proton symporter, where LCFAs remain associated with the transporter via their hydrophobic tails, resulting in an apparent proton transport activated by LCFAs. Related references: PMID:24196960 PMID:28781081
• It regulates the production of reactive oxygen species (ROS) by mitochondria, contributing to cellular redox balance.
• Localized to the mitochondrial inner membrane, UCP1 is a member of the mitochondrial carrier family and contains transmembrane helices characteristic of solute transporters.
• Its expression is restricted to brown adipose tissue, where it is essential for energy expenditure and has implications in obesity and diabetes mellitus.
• Keywords associated with UCP1 include ion transport, oxidation, membrane, and transport, reflecting its role in mitochondrial bioenergetics.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 154-253 of human UCP1, a central region; consider this when mapping epitopes or designing competitive assays.
• For Western blot (WB), the predicted molecular weight is 33 kDa; validate the band size in your sample system and optimize blocking conditions to minimize background.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), ensure proper fixation and antigen retrieval; test various protocols as antigen accessibility can vary across tissues.
• Cross-reactivity with mouse and rat is reported, making this antibody suitable for translational studies in rodent models of thermogenesis; however, always confirm species-specific performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, streamlined for mitochondrial and metabolic disease research. Our sourcing capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-UCP1 reagent, bulk ancillary reagents, and support for rare target raw-material acquisition. Whatever your developmental stage, CamelBio facilitates seamless access to the critical reagents you need to advance from concept to clinic.